Nuclear transport of the major capsid protein is essential for adeno-associated virus capsid formation
Nuclear transport of the major capsid protein is essential for adeno-associated virus capsid formation
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DOI：
10.1128/jvi.73.9.7912-7915.1999
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发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Handa, H
中科院分区：
文献类型：
--
作者：
Hoque, M;Ishizu, K;Handa, H
腺相关病毒衣壳由VP1、VP2和VP3三种蛋白质组成。虽然VP1是病毒感染所必需的，但它不是衣壳形成所必需的。其他衣壳蛋白，VP2和VP3，足以形成衣壳，但每种蛋白的功能作用仍然没有很好地理解。通过对一系列VP2缺失突变体的分析，我们确定了VP2核转移所必需的区域，并发现衣壳蛋白的核定位效率和病毒样颗粒（VLP）形成效率具有良好的相关性。为了证实衣壳蛋白核定位的重要性，我们将猴病毒40大T抗原的核定位信号融合到VP3蛋白上。我们表明该融合蛋白可以形成VLP，这表明位于该蛋白N末端侧的VP 2特异性区域在结构上不需要。这一发现表明，VP3有足够的信息VLP的形成和VP2是必要的，只有核转移的衣壳蛋白。
Adeno-associated virus capsids are composed of three proteins, VP1, VP2, and VP3. Although VP1 is necessary for viral infection, it is not essential for capsid formation. The other capsid proteins, VP2 and VP3, are sufficient for capsid formation, but the functional roles of each protein are still not well understood. By analyzing a series of deletion mutants of VP2, we identified a region necessary for nuclear transfer of VP2 and found that the efficiency of nuclear localization of the capsid proteins and the efficiency of virus-like particle (VLP) formation correlated well. To confirm the importance of the nuclear localization of the capsid proteins, we fused the nuclear localization signal of simian virus 40 large T antigen to VP3 protein. We show that this fusion protein could form VLP, indicating that the VP2-specific region located on the N-terminal side of the protein is not structurally required. This finding suggests that VP3 has sufficient information for VLP formation and that VP2 is necessary only for nuclear transfer of the capsid proteins.