Structural and functional insights into the regulation of the lysis-lysogeny decision in viral communities
Structural and functional insights into the regulation of the lysis-lysogeny decision in viral communities
复制标题
对病毒群落中裂解-溶原决定的调节的结构和功能见解。
DOI：
10.1038/s41564-018-0259-7
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发表时间：
2018-11-01
影响因子：
28.3
通讯作者：
Cheng, Wei
中科院分区：
文献类型：
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作者：
Dou, Chao;Xiong, Jie;Cheng, Wei
通讯对于包括微生物在内的所有生物体都至关重要，细菌群体感应系统清楚地证明了这一点。然而，病毒之间通过群体感应样“仲裁”系统进行通讯的分子机制仍不清楚。已知病毒或宿主密度与溶原性流行率增加有关;然而，从溶原性途径到溶原性途径的转换如何发生尚不清楚。因此，我们试图揭示病毒之间的通信机制，并确定所涉及的溶原动力学。结构和功能分析的噬菌体衍生的SAIRGA和GMPRGA肽和它们相应的受体，phAimR和spAimR，表明SAIRGA通过调节phAimR的构象变化指导phi 3 T的裂解-溶原性决定，而GMPRGA通过稳定二聚体状态的spAimR调节裂解-溶原性途径。虽然温带病毒被认为共享一个类似的溶解-溶原性循环开关模型，我们的研究表明，存在替代菌株特异性机制，调节溶解-溶原性决定。总的来说，这些发现提供了深入了解病毒之间沟通的分子机制，为治疗传染性病毒疾病提供了理论应用。
Communication is vital for all organisms including microorganisms, which is clearly demonstrated by the bacterial quorum-sensing system. However, the molecular mechanisms underlying communication among viruses (phages) via the quorum-sensing-like 'arbitrium' system remain unclear. Viral or host densities are known to be related to an increased prevalence of lysogeny; however, how the switch from the lytic to the lysogenic pathway occurs is unknown. Thus, we sought to reveal mechanisms of communication among viruses and determine the lysogenic dynamics involved. Structural and functional analyses of the phage-derived SAIRGA and GMPRGA peptides and their corresponding receptors, phAimR and spAimR, indicated that SAIRGA directs the lysis-lysogeny decision of phi3T by modulating conformational changes in phAimR, whereas GMPRGA regulates the lysis-lysogeny pathway by stabilizing spAimR in the dimeric state. Although temperate viruses are thought to share a similar lytic-lysogenic cycle switch model, our study suggests the existence of alternative strain-specific mechanisms that regulate the lysis-lysogeny decision. Collectively, these findings provide insights into the molecular mechanisms underlying communication among viruses, offering theoretical applications for the treatment of infectious viral diseases.