Stabilization of islet allografts by treatment of recipients with ultraviolet irradiated donor spleen cells.
Stabilization of islet allografts by treatment of recipients with ultraviolet irradiated donor spleen cells.
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通过用紫外线照射的供体脾细胞处理受体来稳定胰岛同种异体移植物。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
K. Lafferty
中科院分区：
文献类型：
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作者：
M. Agostino;S. Prowse;K. Lafferty
移植前在富氧环境中培养小鼠胰岛可促进移植物长期存活，而无需使用免疫抑制剂。紫外线（UV）照射和供体来源的活脾细胞诱导同种异体移植排斥反应的能力的比较表明，紫外线照射的脾细胞没有免疫原性;活脾细胞具有免疫原性，它们的注射引发同种异体移植排斥反应。从移植后约30天开始用辐照的脾细胞处理后，受体动物能够承受随后用10（6）和10（7）个活供体脾细胞的攻击。当用10（6）个供体脾细胞攻击时，未处理的动物排斥它们的移植物。也就是说，用紫外线照射的细胞处理通过诱导耐受状态来稳定胰岛同种异体移植物。随后移植供体来源和第三方菌株的未培养甲状腺的稳定动物证明耐受性是特异性的。体外免疫反应试验表明，这种耐受性不是由于抗原反应细胞的缺失所致。
Culture of mouse pancreatic islets in an oxygen-rich atmosphere before transplantation facilitates long-term allograft survival without the use of immunosuppression. A comparison of the capacity of ultraviolet (UV) irradiated and live spleen cells of donor origin to induce allograft rejection showed that UV-irradiated spleen cells were not immunogenic; live spleen cells were immunogenic and their injection triggered allograft rejection. Following treatment with irradiated spleen cells from about day 30 post-transplantation, recipient animals were able to withstand subsequent challenges with 10(6) and 10(7) viable donor spleen cells. Untreated animals rejected their graft when challenged with 10(6) donor spleen cells. That is, treatment with UV-irradiated cells stabilized the islet allograft by inducing a state of tolerance. Subsequent transplantation of stabilized animals with uncultured thyroids of both donor origin and from a third party strain demonstrated that the tolerance was specific. In vitro test of immune reactivity showed this tolerance was not due to the deletion of antigen reactive cells.