A Shift toward Smaller Cell Size via Manipulation of Cell Cycle Gene Expression Acts to Smoothen Arabidopsis Leaf Shape1[W]
A Shift toward Smaller Cell Size via Manipulation of Cell Cycle Gene Expression Acts to Smoothen Arabidopsis Leaf Shape1[W]
复制标题
通过操纵细胞周期基因表达向更小的细胞尺寸转变可平滑拟南芥叶片形状1[W]
作者：
Asuka Kuwabara;Andreas Backhaus;R. Malinowski;M. Bauch;L. Hunt;T. Nagata;Nick Monk;G. Sanguinetti;A. Fleming
理解生物体的大小和形状与其组成细胞的大小、形状和数量之间的关系是生物学中的一个基本问题；然而，许多研究表明，这种关系是复杂的，而且往往不是直观的。为了研究这个问题，我们使用了一个系统来诱导表达参与植物细胞周期G1/S转变的基因，并分析了叶片形状的结果。通过结合仔细的发育分期和定量分析细胞分裂模式和叶片形状对这些操作的时空响应，我们发现细胞分裂频率的变化比观察到的叶片形状的变化发生得晚得多。这些数据表明，细胞分裂频率的改变与观察到的形状变化没有因果关系。相反，由于基因操作而导致的细胞尺寸变小与叶片周长更光滑的形成相关，即似乎是主要的细胞驱动因素影响形式。这些数据是在细胞分裂，生长，叶的大小和形状的关系的背景下进行讨论。
Understanding the relationship of the size and shape of an organism to the size, shape, and number of its constituent cells is a basic problem in biology; however, numerous studies indicate that the relationship is complex and often nonintuitive. To investigate this problem, we used a system for the inducible expression of genes involved in the G1/S transition of the plant cell cycle and analyzed the outcome on leaf shape. By combining a careful developmental staging with a quantitative analysis of the temporal and spatial response of cell division pattern and leaf shape to these manipulations, we found that changes in cell division frequency occurred much later than the observed changes in leaf shape. These data indicate that altered cell division frequency cannot be causally involved in the observed change of shape. Rather, a shift to a smaller cell size as a result of the genetic manipulations performed correlated with the formation of a smoother leaf perimeter, i.e. appeared to be the primary cellular driver influencing form. These data are discussed in the context of the relationship of cell division, growth, and leaf size and shape.
影响因子：
10.5
作者：
Umen, JG;Goodenough, UW
通讯作者：
Goodenough, UW