Three-dimensional visualization of plant tissues and organs by X-ray micro-computed tomography
Three-dimensional visualization of plant tissues and organs by X-ray micro-computed tomography
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X 射线微计算机断层扫描对植物组织和器官的三维可视化
DOI：
10.1093/jmicro/dfad026
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发表时间：
2023
期刊：
影响因子：
1.8
通讯作者：
Mineyuki Y
中科院分区：
文献类型：
--
作者：
Karahara I;Yamauchi D;Uesugi K;Mineyuki Y
回顾了自2015年以来发表的利用微型计算机断层扫描(CT)在三维(3D)上可视化植物组织和器官的研究。在此期间，随着以实验室为基础的高性能微型CT系统的发展以及同步辐射设施尖端技术的不断发展，涉及微型CT的植物科学领域的出版物数量有所增加。商用的基于实验室的微型CT系统的广泛使用实现了相衬成像技术，这种技术适合于对由轻元素组成的生物标本进行可视化，似乎促进了这些研究。植物体内的独特特征，特别是用于通过Micro-CT对植物器官和组织进行成像的特征，具有功能空间和特殊的细胞壁，如木质化的细胞壁。本文首先简要介绍了Micro-CT技术的基本原理，然后详细介绍了它在植物科学中的三维可视化应用，包括各种器官、颖果、种子、其他器官(生殖器官、叶、茎和叶柄)、各种组织(叶脉、木质部、充气组织、细胞边界、细胞壁)、栓塞物和根系的成像，希望广大显微镜和其他成像技术的用户也能对Micro-CT感兴趣，从而为更深入地了解植物组织和器官的三维结构提供一些启示。目前使用微CT进行的形态研究大多仍处于定性水平。为了使未来的研究从定性水平过渡到定量水平，需要发展精确的三维分割方法学。
Studies visualizing plant tissues and organs in three-dimension (3D) using micro–computed tomography (CT) published since approximately 2015 are reviewed. In this period, the number of publications in the field of plant sciences dealing with micro-CT has increased along with the development of high-performance lab-based micro-CT systems as well as the continuous development of cutting-edge technologies at synchrotron radiation facilities. The widespread use of commercially available lab-based micro-CT systems enabling phase-contrast imaging technique, which is suitable for the visualization of biological specimens composed of light elements, appears to have facilitated these studies. Unique features of the plant body, which are particularly utilized for the imaging of plant organs and tissues by micro-CT, are having functional air spaces and specialized cell walls, such as lignified ones. In this review, we briefly describe the basis of micro-CT technology first and then get down into details of its application in 3D visualization in plant sciences, which are categorized as follows: imaging of various organs, caryopses, seeds, other organs (reproductive organs, leaves, stems and petioles), various tissues (leaf venations, xylems, air-filled tissues, cell boundaries, cell walls), embolisms and root systems, hoping that wide users of microscopes and other imaging technologies will be interested also in micro-CT and obtain some hints for a deeper understanding of the structure of plant tissues and organs in 3D. Majority of the current morphological studies using micro-CT still appear to be at a qualitative level. Development of methodology for accurate 3D segmentation is needed for the transition of the studies from a qualitative level to a quantitative level in the future.