NEURAMINIDASE-INDUCED THROMBOCYTOPENIA IN RATS
NEURAMINIDASE-INDUCED THROMBOCYTOPENIA IN RATS
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DOI：
10.1111/j.1365-2141.1972.tb08790.x
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发表时间：
1972-01-01
影响因子：
6.5
通讯作者：
JOURNEY, LJ
中科院分区：
文献类型：
--
作者：
CHOI, SI;SIMONE, JV;JOURNEY, LJ
本研究的目的是阐明神经氨酸酶对大鼠循环血小板的影响及其机制。注射细菌、纯化细菌和病毒神经氨酸酶引起不同程度的血小板减少。在给予病毒神经氨酸酶的大鼠和脾切除动物中，血小板减少症不太明显。仅在静脉注射纯化细菌神经氨酸酶时观察到剂量反应效应。注射神经氨酸酶后，进行血小板超微结构研究。注射神经氨酸酶后15分钟，在计数明确下降之前，血小板显示出结构损伤的证据，如形状不规则、向心迁移和颗粒融合以及滤过泡形成。结构改变是进行性的，在约2-4小时达到最大值。在循环血液中未观察到大的血小板聚集体。注射神经氨酸酶后4小时，血小板计数明显下降，许多血小板超微结构正常。到24 h时，大部分循环血小板显示正常;然而，脾窦和巨噬细胞含有与注射神经氨酸酶后15 min至2 h在循环中观察到的结构异常相同的血小板。我们的结论是，神经氨酸酶改变血小板膜，导致其快速和永久性的网状内皮系统清除。
The purpose of this study was to elucidate the effect of neuraminidase on circulating platelets in rats and the pathogenesis of this effect. Injection of bacterial, purified bacterial and viral neuraminidase caused thrombocytopenia of various degrees. Thrombocytopenia was less pronounced in rats given viral neuraminidase and in splenectomized animals. A dose‐response effect was seen only with purified bacterial neuraminidase injected intravenously. Ultrastructural studies of blood platelets were performed after injection of neuraminidase. Fifteen minutes after injecting neuraminidase, before a definite fall in the count, platelets showed evidence of structural damage such as irregular shapes, centripetal migration and fusion of granules and bleb formation. The structural alterations were progressive, reaching a maximum at about 2–4 hr. At no time were large platelet aggregates observed in the circulating blood. Four hours after injection of neuraminidase, when the platelet count dropped significantly, many platelets had normal ultrastructurc. By 24 hr, the majority of circulating platelets appeared normal; however, the splenic sinusoids and macrophages contained platelets with the same structural abnormalities seen in the circulation 15 min to 2 hr after injection of neuraminidase. We conclude that neuraminidase alters the platelet membrane resulting in its rapid and permanent removal by the reticuloendothelial system.