Identification of Oxidized Amino Acid Residues in the Vicinity of the Mn4CaO5 Cluster of Photosystem II: Implications for the Identification of Oxygen Channels within the Photosystem
Identification of Oxidized Amino Acid Residues in the Vicinity of the Mn4CaO5 Cluster of Photosystem II: Implications for the Identification of Oxygen Channels within the Photosystem
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DOI：
10.1021/bi300650n
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发表时间：
2012-08-14
期刊：
影响因子：
2.9
通讯作者：
Bricker, Terry M.
中科院分区：
文献类型：
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作者：
Frankel, Laurie K.;Sallans, Larry;Bricker, Terry M.
作为一种光驱动的水-质体醌氧化还原酶，Photosystem II 产生分子氧作为酶产物。此外，在各种应激条件下，在活性位点处或附近会产生活性氧物质以释放氧气。在本研究中，使用傅里叶变换离子回旋共振质谱法来鉴定从菠菜光系统 II 膜分离的几个核心光系统 II 蛋白（D1、D2、CP43 和 CP47）中的氧化氨基酸残基。虽然这些氧化残基中的大多数 (81%) 位于复合物暴露于含氧溶剂的表面上，但 CP43 蛋白上的几个残基（E-354、T-355、M-356 和 R-357）非常接近（
As a light-driven water-plastoquinone oxidoreductase, Photosystem II produces molecular oxygen as an enzymatic product. Additionally, under a variety of stress conditions, reactive oxygen species are produced at or near the active site for oxygen evolution. In this study, Fourier-transform ion cyclotron resonance mass spectrometry was used to identify oxidized amino acid residues located in several core Photosystem II proteins (D1, D2, CP43, and CP47) isolated from spinach Photosystem II membranes. While the majority of these oxidized residues (81%) are located on the oxygenated solvent-exposed surface of the complex, several residues on the CP43 protein (E-354, T-355, M-356, and R-357) which are in close proximity (