The air-gap PAD: a roll-to-roll-compatible fabrication method for paper microfluidics
The air-gap PAD: a roll-to-roll-compatible fabrication method for paper microfluidics
复制标题
气隙 PAD：纸微流体的卷对卷兼容制造方法
DOI：
10.1039/d2lc01164f
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发表时间：
2023
期刊：
影响因子：
6.1
通讯作者：
Lieberman, Marya
中科院分区：
文献类型：
--
作者：
Roller, Rachel M.;Rea, Angela;Lieberman, Marya
纸基分析设备（PAD）为快速使用点测试提供了一个低成本、用户友好的平台。然而，如果没有可扩展的制造方法，很少有PAD能够走出学术实验室并进入最终用户手中。以前，蜡印刷被认为是理想的PAD制造方法，但考虑到蜡印刷机不再商业化，需要替代品。在这里，我们提出了一个这样的替代方案：气隙PAD。气隙PAD由亲水性纸测试区组成，由“气隙”分隔，并用双面胶固定在疏水性背衬上。这种设计的主要吸引力是它与用于大规模制造的卷对卷设备的兼容性。在这项研究中，我们研究气隙PAD的设计考虑，比较蜡印和气隙PAD的性能，并报告中试规模的卷到卷的生产运行的气隙PAD的合作伙伴关系与商业测试条制造商。在Washburn流动实验、纸质滴定和12道药物筛选装置中，气隙装置的性能与蜡印装置相当。使用卷到卷制造，我们生产了2700英尺的气隙PAD，每个PAD仅需0.03美元。
Paper-based analytical devices (PADs) offer a low-cost, user-friendly platform for rapid point-of-use testing. Without scalable fabrication methods, however, few PADs make it out of the academic laboratory and into the hands of end users. Previously, wax printing was considered an ideal PAD fabrication method, but given that wax printers are no longer commercially available, alternatives are needed. Here, we present one such alternative: the air-gap PAD. Air-gap PADs consist of hydrophilic paper test zones, separated by “air gaps” and affixed to a hydrophobic backing with double-sided adhesive. The primary appeal of this design is its compatibility with roll-to-roll equipment for large-scale manufacturing. In this study, we examine design considerations for air-gap PADs, compare the performance of wax-printed and air-gap PADs, and report on a pilot-scale roll-to-roll production run of air-gap PADs in partnership with a commercial test-strip manufacturer. Air-gap devices performed comparably to their wax-printed counterparts in Washburn flow experiments, a paper-based titration, and a 12-lane pharmaceutical screening device. Using roll-to-roll manufacturing, we produced 2700 feet of air-gap PADs for as little as $0.03 per PAD.