Intraperitoneal photodynamic therapy for an ovarian cancer ascite model in Fischer 344 rat using hematoporphyrin monomethyl ether
Intraperitoneal photodynamic therapy for an ovarian cancer ascite model in Fischer 344 rat using hematoporphyrin monomethyl ether
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DOI：
10.1111/j.1349-7006.2007.00628.x
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发表时间：
2007-12
期刊：
影响因子：
5.7
通讯作者：
K. Song;B. Kong;Li Li-Li;Qifeng Yang;Yongqing Wei;X. Qu
中科院分区：
文献类型：
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作者：
K. Song;B. Kong;Li Li-Li;Qifeng Yang;Yongqing Wei;X. Qu
由于治疗方案有限，卵巢癌腹腔内扩散是导致高发病率的常见问题。腹膜内光动力疗法联合减容手术治疗残留病变是临床医生的另一种选择。血卟啉单甲基醚（HMME）是国内开发的一种很有前途的第二代光敏剂。本研究旨在探讨HMME对卵巢癌的光毒性。NuTu - 19是来源于Fischer 344大鼠腺癌的细胞系，其异体移植腹水肿瘤模型被用于本研究。HMME被证实定位于细胞溶酶体中，基于HMME的光敏诱导直接坏死和线粒体损伤。HMME的光细胞毒性与光和药物剂量均相关，在NuTu - 19细胞中未观察到明显的暗细胞毒性。在Fischer 344腹水肿瘤大鼠模型中，基于HMME的腹腔光动力疗法被证明有助于改善卵巢癌的预后。因此，本研究为基于HMME的光动力疗法是卵巢癌有效的辅助疗法提供了证据。（癌症科学2007；98:1959-1964）
With limited treatment options, intraperitoneal spread of ovarian cancer is a common problem leading to high morbidity. Intraperitoneal photodynamic therapy combined with debulking surgery to treat residual disease is an alternative choice for clinicians. Hematoporphyrin monomethyl ether (HMME) is a promising second‐generation photosensitizer developed in China. Our study was designed to investigate the phototoxicity of HMME on ovarian cancer. NuTu‐19, a cell line derived from adenocarcinoma of Fischer 344 rat, and its allogeneic graft ascites tumor model was used in this study. HMME was confirmed to be localized in cytolysosome, and HMME‐based photosensitization induced direct necrosis as well as mitochondria damage. The photocytotoxicity of HMME was both light‐ and drug dose‐dependent and no significant dark cytotoxicity was observed in NuTu‐19 cells. With the ascite tumor‐bearing Fischer 344 rat model, HMME‐based intraperitoneal photodynamic therapy was proved to be useful in improving the prognosis of ovarian cancer. Thus, this study provides evidence that HMME‐based photodynamic therapy is an effective adjuvant therapy for ovarian cancer. (Cancer Sci 2007; 98: 1959–1964)