Quantification of atherosclerotic plaque composition in cholesterol-fed rabbits with 50-MHz acoustic microscopy.
Quantification of atherosclerotic plaque composition in cholesterol-fed rabbits with 50-MHz acoustic microscopy.
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使用 50 MHz 声学显微镜对胆固醇喂养的兔子中的动脉粥样硬化斑块成分进行定量。
DOI：
10.1161/01.atv.12.10.1227
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Wickline,SA
中科院分区：
文献类型：
--
作者：
Shepard,RK;Miller,JG;Wickline,SA
为了确定高频超声是否可以区分正常和病理性血管结构，并阐明正常和动脉粥样硬化动脉的不同层中超声背向散射的决定因素，使用50 MHz的高分辨率声学显微镜来表征6只新西兰白色家兔（喂食2%胆固醇饮食3.5个月）的主动脉斑块。4只家兔饲喂标准饲料3.5个月，以提供正常对照数据。切下腹主动脉节段，固定在福尔马林中，纵向打开，并平放进行声穿透。对于每个标本，背散射射频（rf）数据采集从30至100个独立的网站分开500微米。从血管壁的离散层门控射频数据的一部分，用于计算背向散射积分。血管壁厚度和成分的组织学和免疫细胞化学分析结果与超声分析结果进行了比较。正常的动脉瘤在整个血管壁表现出明显但均匀的背向散射（平均背向散射积分，-28.5 +/- 2.9 dB），内膜和中层之间没有明显的区别。动脉粥样硬化动脉瘤表现为增厚内膜的背向散射积分显著降低（-47.5 +/-3.2 dB，p < 0.0001），但中膜的背向散射积分相对正常（-31.2 +/-1.6 dB; p = NS vs正常动脉瘤）。正常和动脉粥样硬化家兔的中膜厚度约为300微米。动脉粥样硬化性动脉瘤的组织学特征证实存在大量内膜增厚，伴明显的泡沫细胞和脂质浸润，邻接更正常的中层。（250字处删节）
To determine whether high-frequency ultrasound could distinguish normal from pathological vascular structure and to elucidate the determinants of ultrasonic backscatter in different layers of normal and atherosclerotic arteries, high-resolution acoustic microscopy at 50 MHz was used to characterize aortic plaque in six New Zealand White rabbits fed a 2% cholesterol diet for 3.5 months. Four rabbits were fed a standard diet for 3.5 months to provide normal control data. Segments of aortas were excised, fixed in formalin, opened longitudinally, and mounted flat for insonification. For each specimen, backscattered radio frequency (rf) data were acquired from 30 to 100 independent sites separated by 500 microns. Portions of rf data were gated from discrete layers of the vessel wall for computation of integrated backscatter. Results of histological and immunocytochemical analyses of vessel wall thickness and composition were compared with those of ultrasonic analysis. Normal aortas manifested prominent but homogeneous backscatter (average integrated backscatter, -28.5 +/- 2.9 dB) throughout the vessel wall, with no clear distinction between intimal and medial layers. The atherosclerotic aortas manifested substantially reduced integrated backscatter from the thickened intima (-47.5 +/- 3.2 dB, p < 0.0001) but relatively normal integrated backscatter from the media (-31.2 +/- 1.6 dB; p = NS versus normal aortas). The thickness of the media for both normal and atherosclerotic rabbits was approximately 300 microns. Histological characteristics of atherosclerotic aortas confirmed the presence of substantial intimal thickening, with prominent foam cell and lipid infiltration abutting a more normal medial layer.(ABSTRACT TRUNCATED AT 250 WORDS)