Halothane attenuation of muscarinic inhibition of adenylate cyclase in rat heart.
Halothane attenuation of muscarinic inhibition of adenylate cyclase in rat heart.
复制标题
氟烷减弱大鼠心脏腺苷酸环化酶的毒蕈碱抑制作用。
DOI：
10.1016/0006-2952(88)90774-5
复制
发表时间：
1988
影响因子：
5.8
通讯作者：
Aronstam,RS
中科院分区：
文献类型：
--
作者：
Narayanan,TK;Confer,RA;DennisonJr,RL;Anthony,BL;Aronstam,RS
氟烷刺激大鼠心肌细胞膜腺苷酸环化酶活性。用2%氟烷平衡膜后获得最大刺激（54%）。氟烷不影响由毛喉素或异丙肾上腺素产生的腺苷酸环化酶活性的刺激分数。然而，氟烷减少氨甲酰胆碱抑制腺苷酸环化酶活性刺激毛喉素和异丙肾上腺素。氨甲酰胆碱抑制刺激环化酶活性的最大抑郁症后，得到平衡与1%氟烷。这些结果与配体结合研究的证据一致，并表明氟烷破坏了毒蕈碱受体与G蛋白的相互作用。
Halothane stimulated basal adenylate cyclase activity in rat cardiac membranes. Maximal stimulation (54%) was obtained after equilibrating the membranes with 2% halothane. Halothane did not affect the fractional stimulation of adenylate cyclase activity produced by either forskolin or isoproterenol. However, halothane decreased carbamylcholine inhibition of adenylate cyclase activity stimulated by both forskolin and isoproterenol. Maximal depression of carbamylcholine inhibition of stimulated cyclase activity was obtained after equilibration with 1% halothane. These results are consistent with evidence from ligand binding studies and indicate that halothane disrupts muscarinic receptor-G-protein interactions.