Glycosylation of urinary prostate-specific antigen in benign hyperplasia and cancer: assessment by lectin-binding patterns
Glycosylation of urinary prostate-specific antigen in benign hyperplasia and cancer: assessment by lectin-binding patterns
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DOI：
10.1016/j.clinbiochem.2004.09.022
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Kosanovic, MM
中科院分区：
文献类型：
--
作者：
Jankovic, MM;Kosanovic, MM
目的：在本研究中，我们研究了糖基化的尿前列腺特异性抗原（PSA）从良性前列腺增生（BPH）和前列腺癌（PCa）的科目，特别是在它的寡糖链的改变，作为一个潜在的生物标志物，这些pathophysiological conditions.Design和方法：第一个早晨尿液收集从受试者与前列腺癌和BPH开始任何治疗前。尿PSA的特征在于通过离子交换色谱，然后通过凝集素亲和层析柱上使用固定化plantlectins.Results的柱：4个异构体的尿PSA从BPH和PCa样品分离的离子交换色谱。从凝集素亲和柱的洗脱曲线反映了PSA亚型的分子异质性，观察到的主要差异是在反应性荆豆applutinin，Aleuria aurantia凝集素，菜豆红细胞凝集素和菜豆leukoagglutininin.Conclusions：在BPH PSA和前列腺癌PSA之间的凝集素反应性观察到的差异可能是在临床上的重要性，在前列腺健康的评价。(C)2004年加拿大临床化学家协会。All rights reserved.
Objectives: In the present study, we examined the glycosylation of urinary prostate-specific antigen (PSA) from benign prostatic hyperplasia (BPH) and prostate cancer (PCa) subjects, specifically looking at alterations in it oligosaccharide chain as a potential biomarker of these pathophysiological conditions.Design and methods: First morning urine voide were collected from subjects with PCa and BPH before initiation of any treatment. Urinary PSA was characterized by ion-exchange chromatography, followed by lectin affinity chromatography on the columns using immobilized plant lectins.Results: Four isoforms of urinary PSA from both BPH and PCa samples were separated by ion-exchange chromatography. The elution profiles from lectin-affinity columns reflected molecular heterogeneity of PSA isoforms and the main differences observed were in the reactivity to Ulex europaeus applutinin, Aleuria aurantia agglutinin, Phaseolus vulgaris erythroagglutinin and Phaseolus vulgaris leukoagglutinin.Conclusions: The observed differences in the lectin reactivities between BPH PSA and PCa PSA may be of clinical importance in the evaluation of prostate health. (C) 2004 The Canadian Society of Clinical Chemists. All rights reserved.