Molecular Genetic and Chemotaxonomic Identification of the Bacterium of the Genus Ochrobactrum Possessing Oil-Oxidizing and Nitrogen-Fixing Activity
Molecular Genetic and Chemotaxonomic Identification of the Bacterium of the Genus Ochrobactrum Possessing Oil-Oxidizing and Nitrogen-Fixing Activity
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DOI：
10.1134/s1062359017050090
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发表时间：
2017-09-01
期刊：
影响因子：
0.5
通讯作者：
Loginov, O. N.
中科院分区：
文献类型：
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作者：
Korshunova, T. Yu.;Mukhamatdyarova, S. R.;Loginov, O. N.
结果表明，采用气相色谱-质谱联用分析细胞脂肪酸和应用编码DNA旋切酶β亚基的基因作为替代系统发育标记补充了具有生物技术价值的菌株Ochrobactrum sp. IB DT-5.3/2的分类描述，但对细菌的物种鉴定无效。然而，根据蛋白质组分的基质辅助激光解吸-电离飞行时间质谱分析结果和我们之前的培养形态学和生化特征研究，以及16S rRNA基因测序，我们可以得出结论，菌株Ochrobactrum sp. IB DT-5.3/2为O. intermedium。
It was shown that analysis of cellular fatty acids using gas chromatography-mass-spectrometry and application of the gene encoding beta-subunit of DNA gyrase (gyrB) as an alternative phylogenetic marker supplemented the taxonomic description of the biotechnologically valuable strain Ochrobactrum sp. IB DT-5.3/2 but were ineffective for species identification of the bacterium. However, based on the results of the matrix-assisted laser desorption-ionization time of flight mass spectrometry of the protein fractions and our previous studies of the cultural-morphological and biochemical features, as well as sequencing of the 16S rRNA gene, we can conclude that the strain Ochrobactrum sp. IB DT-5.3/2 refers to the species O. intermedium.