Tamarind seed coat extract restores reactive oxygen species through attenuation of glutathione level and antioxidant enzyme expression in human skin fibroblasts in response to oxidative stress
Tamarind seed coat extract restores reactive oxygen species through attenuation of glutathione level and antioxidant enzyme expression in human skin fibroblasts in response to oxidative stress
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DOI：
10.12980/apjtb.4.2014c806
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发表时间：
2014-05-01
影响因子：
1.7
通讯作者：
Pongsamart, Sunanta
中科院分区：
文献类型：
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作者：
Nakchat, Oranuch;Nalinratana, Nonthaneth;Pongsamart, Sunanta
目的：探讨罗望子种皮提取物（TSCE）对正常和过氧化氢（H2O2）诱导的氧化应激条件下正常人皮肤成纤维细胞CCD-1064Sk细胞的作用和机制。方法：用沸水提取罗望子种皮，然后用乙酸乙酯分配，然后进行细胞分析。研究了 TSCE 对细胞内活性氧 (ROS)、谷胱甘肽 (GSH) 水平、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GPx) 等抗氧化酶和过氧化氢酶活性（包括抗氧化蛋白表达）的影响。结果：TSCE 通过增加 GSH 水平，在不存在和存在 H2O2 或 H2O2 的情况下显着减弱细胞内 ROS。在没有 H2O2 的情况下，TSCE 显着增强 SOD 和过氧化氢酶活性，但对 GPx 没有影响。同时，TSCE显着增加H2O2处理细胞中SOD和GPx的蛋白表达。结论：TSCE通过清除SOS、降低GSH水平而表现出抗氧化活性，从而保护人皮肤成纤维细胞免受氧化应激。我们的研究结果强调了罗望子籽煤通过抗氧化酶系统的抗氧化机制，该提取物对罗望子籽皮的保健食品和药妆品应用具有潜在的益处。
Objective: To investigate the role and mechanism of tamarind seed coat extract (TSCE) on normal human skin fibroblast CCD-1064Sk cells under normal and oxidative stress conditions induced by hydrogen peroxide (H2O2).Methods: Tamarind seed coats were extracted with boiling water and then partitioned with ethyl acetate before the cell analysis. Effect of TSCE on intracellular reactive oxygen species (ROS), glutathione (GSH) level, antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalasc activity including antioxidant protein expression was investigated.Results: TSCE significantly attenuated intracellular ROS in the absence and presence or H2O2 by increasing GSH level. In the absence of H2O2, TSCE significantly enhanced SOD and catalase activity but did not affected on GPx. Meanwhile, TSCE significantly increased the protein expression of SOD and GPx in H2O2 treated cells.Conclusions: TSCE exhibited antioxidant activities by scavenging SOS, attenuating GSH level that could protect human skin fibroblast cells from oxidative stress. Our results highlight the antioxidant mechanism of tamarind seed coal through an antioxidant enzyme system, the extract potentially benefits for health food and cosmeceutical application of tamarind seed coat.