Effects of follicular atresia and size on the developmental competence of bovine oocytes: A study using the well-in-drop culture system
Effects of follicular atresia and size on the developmental competence of bovine oocytes: A study using the well-in-drop culture system
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DOI：
10.1016/j.theriogenology.2007.01.017
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发表时间：
2007-05-01
期刊：
影响因子：
2.8
通讯作者：
Tan, J.-H.
中科院分区：
文献类型：
--
作者：
Feng, W.-G.;Sui, H.-S.;Tan, J.-H.
采用滴孔式卵母细胞/胚胎培养系统研究了颗粒细胞（GC）凋亡和卵泡大小对牛卵母细胞能力的影响。在WID系统中培养的卵母细胞的囊胚孵化率（13%）与在常规组系统中培养的卵母细胞的囊胚孵化率（16%）无显著差异（P > 0.05）。闭锁早期囊胚孵化率（17%）显著高于非闭锁卵泡（8%）和闭锁晚期卵泡（10%）（P < 0.05）;闭锁早期6-8 mm卵泡孵化率（22%）显著高于4-5 mm卵泡孵化率（15%）。相同大小（4-8 mm）的晚期闭锁卵泡卵母细胞（17%）比非闭锁卵泡卵母细胞（7%）或早期闭锁卵泡卵母细胞（9%）更多（P < 0.05）被阻滞在I级卵丘扩张（仅外周两层细胞开始扩张）。同样，在闭锁晚期，2 ~ 3 mm卵泡的卵母细胞（30%）比6 ~ 8 mm卵泡的卵母细胞（21%）具有更多的I级卵丘扩张（P < 0.05）。早期闭锁卵泡中3级（除辐射状细胞外的所有卵丘层均扩张）或4级（完全）卵丘扩张的卵母细胞的孵化囊胚率（20%）高于非闭锁卵泡（13%）或晚期闭锁卵泡（12%）。早期闭锁卵泡卵母细胞的囊胚孵化率随卵丘从2级（9%）扩大到4级（27%）而增加。无论卵泡闭锁的程度如何，卵泡液（FF）中72-76%的漂浮细胞正在经历凋亡。FF中的漂浮细胞密度与卵母细胞的发育潜能高度相关（r = 0.6-0.7）。总之，WID培养系统与组培养一样有效，并允许鉴定卵泡来源。此外，卵母细胞的发育潜能受GC凋亡、卵泡大小和卵丘扩张的影响，FF中的漂浮细胞密度可作为卵母细胞质量的一个简单、无创的指标。(c)2007年爱思唯尔公司所有的战斗保留。
The effect of granulosa cell (GC) apoptosis and follicle size on the competence of bovine oocytes were studied using a well-in-drop (WID) oocyte/embryo culture system, which allows identification of follicular origin. Hatching rates of blastocysts did not differ (P > 0.05) between oocytes cultured in the WID system (13%) and those cultured in the conventional group system (16%). Hatching rates of blastocysts were higher (P < 0.05) in early atretic (17%) than in non-atretic (8%) and late atretic follicles (10%) of the same size (4-8 mm), and in 6-8 mm (22%) than in 4-5 mm follicles (15%) at the early atretic stage. More oocytes (P < 0.05) from late atretic (17%) than from non-atreteic (7%) or early atretic follicles (9%) of the same size (4-8 mm) were arrested at Grade I cumulus expansion (only cells in the peripheral two layers began to expand). Similarly, more oocytes from 2 to 3 mm follicles (30%) than from 6 to 8 mm follicles (21%) at the same (late) atretic stage had Grade I cumulus expansion (P < 0.05). Hatching blastocyst percentages of oocytes with Grade 3 (all layers of the cumulus except corona radiate cells expanded) or Grade 4 (full) cumulus expansion were higher in early atretic (20%) than in non-atretic (13%) or late atretic follicles (12%). Hatching blastocyst percentages of oocytes from follicles at the early atretic stage increased as cumulus expanded from Grade 2 (9%) to Grade 4 (27%). Regardless of the degree of follicle atresia, 72-76% of the floating cells in the follicular fluid (FF) were undergoing apoptosis. The floating cell density in FF was highly (r = 0.6-0.7) correlated with oocyte developmental potency. In conclusion, the WID culture system was as efficient as group culture and allowed identification of follicular origin. Furthermore, the developmental potential of oocytes was affected by GC apoptosis, follicle size and cumulus expansion, and the floating cell density in FF could be used as a simple and non-invasive marker of oocyte quality. (c) 2007 Elsevier Inc. All fights reserved.