Application of mass spectrometry to the discovery of biomarkers for detection of prostate cancer
Application of mass spectrometry to the discovery of biomarkers for detection of prostate cancer
复制标题
DOI：
10.1002/jcb.20855
复制
发表时间：
2006-06-01
影响因子：
4
通讯作者：
Ward, Mike
中科院分区：
文献类型：
--
作者：
Semmes, O. John;Malik, Gunjan;Ward, Mike
已经出现了一个令人印象深刻的基于质谱的技术应用于蛋白质的研究。同样值得注意的是，这些技术迅速适应临床蛋白质组学领域的生物医学方法。致力于阐明器官部位或特定疾病状态的蛋白质组的协同努力正在激增，并且从这些努力中获得了更好的诊断/诊断学和治疗干预的希望。前列腺癌一直是许多此类研究的焦点，通过这些蛋白质组学方法获得的生物标志物有望改善对患者的护理。新技术提供更高的分析能力，采用自动化液体处理系统，分馏技术和生物信息学工具，以获得更高的灵敏度和分辨率，更强大和更高通量的样品处理，以及更大的分析结果的信心。本文就蛋白质组学技术在前列腺癌研究中的应用进行了综述，沿着介绍了其各自的优缺点。新的蛋白质组学策略的发展在未来的应用进行了讨论。J.细胞。98：496-503，2006. (c)2006 Wiley-Liss，Inc.
There has been an impressive emergence of mass spectrometry based technologies applied toward the study of proteins. Equally notable is the rapid adaptation of these technologies to biomedical approaches in the realm of clinical proteomics. Concerted efforts toward the elucidation of the proteomes of organ sites or specific disease state are proliferating and from these efforts come the promise of better diagnostics/prognostics and therapeutic intervention. Prostate cancer has been a focus of many such Studies with the Promise of improved care to patients via biomarkers derived from these proteomic approaches. The newer technologies provide higher analytical capabilities, employ automated liquid handling systems, fractionation techniques and bioinformatics tools for greater sensitivity and resolving power, more robust and higher throughput sample processing, and greater confidence in analytical results. In this prospects, we summarize the proteomic technologies applied to date in Prostate cancer, along with their respective advantages and disadvantages. The development of newer proteomic strategies for use in future applications is also discussed. J. Cell. Biochem. 98: 496-503, 2006. (c) 2006 Wiley-Liss, Inc.