Comparative analysis of transforming growth factor-beta isoforms 1-3 in human and rabbit dentine matrices
Comparative analysis of transforming growth factor-beta isoforms 1-3 in human and rabbit dentine matrices
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DOI：
10.1016/s0003-9969(96)00115-x
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发表时间：
1997-03-01
影响因子：
3
通讯作者：
Smith, AJ
中科院分区：
文献类型：
--
作者：
Cassidy, N;Fahey, M;Smith, AJ
以前的研究表明，转化生长因子β（TGF-β）与牙齿发育有关。在这里，分析了牙本质细胞外基质中的TGF-β亚型，因为这些分子可能参与牙齿组织修复。EDTA-可溶性和胶原酶释放的馏分从人冠和根和兔切牙牙本质样品中分离，并分析TGF-β亚型。TGF-β（1）是在所有样本中检测到的主要同种型，并且是在人类牙本质样本中检测到的唯一同种型。TGF-β（2）仅在兔切牙牙本质的胶原酶释放部分中检测到，且水平较低。在兔牙本质的EDTA可溶性和胶原酶释放的组分中都检测到TGF-β 3。在兔牙本质样本中检测到的TGF-β（1）亚型水平高于人牙本质样本，并且在这两个物种之间观察到两种组织组分之间的分布存在一些差异。牙本质中TGF-β的这些亚型的存在可能在基质中提供生长因子的储存库，其可以参与导致损伤后的组织修复的过程。(C)1997年爱思唯尔科学有限公司
Previous studies have implicated transforming growth factor-beta(s)(TGF-beta) in tooth development. Here TGF-beta isoforms in dentine extracellular matrix were analysed because these molecules may participate in dental tissue repair. EDTA-soluble and collagenase-released fractions were isolated from human crown and root and rabbit incisor dentine samples and analysed for TGF-beta isoforms. TGF-beta(1) was the major isoform detected in all samples and the only isoform detected in human dentine samples. TGF-beta(2) was detected only in the collagenase-released fraction of rabbit incisor dentine and was present at low levels. TGF-beta(3) was detected in both EDTA-soluble and collagenase-released fractions of rabbit dentine. Greater levels of the TGF-beta(1) isoform were detected in the rabbit than human dentine samples and some differences in distribution amongst the two tissue fractions were observed between these species. The presence of these isoforms of TGF-beta in dentine may provide a reservoir of growth factor in the matrix that could participate in processes leading to tissue repair after injury. (C) 1997 Elsevier Science Ltd.