Cytoarchitecture, areas, and neuron numbers of the Etruscan Shrew cortex
Cytoarchitecture, areas, and neuron numbers of the Etruscan Shrew cortex
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DOI：
10.1002/cne.23053
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发表时间：
2012-08-01
影响因子：
2.5
通讯作者：
Brecht, M.
中科院分区：
文献类型：
--
作者：
Naumann, R. K.;Anjum, F.;Brecht, M.
Etruscan 鼩，Suncus etruscus，是最小的哺乳动物之一。伊特鲁里亚鼩能够以惊人的速度和准确性识别猎物的形状，基于胡须介导的触觉线索。由于其体积小，定量分析伊特鲁里亚鼩皮层比其他动物更容易处理。为了定量评估伊特鲁里亚鼩的大脑解剖结构，我们将大脑切片，并应用尼氏染色和NeuN（神经元核）抗体染色。在这些染色的基础上，我们用Stereoinvestigator和Neurolucida（MBF Bioscience）软件估计了10个皮质半球的神经元数量。平均而言，每个半球的神经元数量为100万。我们还测量了皮质表面积，发现每个半球的平均面积为11.1 mm2（n = 7），平均体积为5.3 mm3（n = 10）。我们确定了13个皮质区域的cytoarchitectonic边界在冠状面，矢状面和切向截面处理尼氏物质，髓鞘，细胞色素氧化酶，离子锌，神经丝，和囊泡谷氨酸转运蛋白2（VGluT2）。伊特鲁里亚鼩是一种具有高度触觉的动物，有一个大的躯体感觉皮层，其中包含一个桶场，但桶场的定义远不如啮齿动物清晰。解剖学推导的皮层分区方案大致对应于生理学推导的新皮层感觉区的地图。神经学比较杂志520：25122530，2012年。(C)2012 Wiley Periodicals，Inc.
The Etruscan shrew, Suncus etruscus, is one of the smallest mammals. Etruscan shrews can recognize prey shape with amazing speed and accuracy, based on whisker-mediated tactile cues. Because of its small size, quantitative analysis of the Etruscan shrew cortex is more tractable than in other animals. To quantitatively assess the anatomy of the Etruscan shrew's brain, we sectioned brains and applied Nissl staining and NeuN (neuronal nuclei) antibody staining. On the basis of these stains, we estimated the number of neurons of 10 cortical hemispheres by using Stereoinvestigator and Neurolucida (MBF Bioscience) software. On average, the neuron number per hemisphere was found to be 1 million. We also measured cortical surface area and found an average of 11.1 mm2 (n = 7) and an average volume of 5.3 mm3 (n = 10) per hemisphere. We identified 13 cortical regions by cytoarchitectonic boundaries in coronal, sagittal, and tangential sections processed for Nissl substance, myelin, cytochrome oxidase, ionic zinc, neurofilaments, and vesicular glutamate transporter 2 (VGluT2). The Etruscan shrew is a highly tactile animal with a large somatosensory cortex, which contains a barrel field, but the barrels are much less clearly defined than in rodents. The anatomically derived cortical partitioning scheme roughly corresponds to physiologically derived maps of neocortical sensory areas. J. Comp. Neurol. 520:25122530, 2012. (C) 2012 Wiley Periodicals, Inc.