KILLIFISH OPERCULAR SKIN - FLAT EPITHELIUM WITH A HIGH-DENSITY OF CHLORIDE CELLS
KILLIFISH OPERCULAR SKIN - FLAT EPITHELIUM WITH A HIGH-DENSITY OF CHLORIDE CELLS
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DOI：
10.1002/jez.1401990309
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
KINTER, WB
中科院分区：
文献类型：
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作者：
KARNAKY, KJ;KINTER, WB
在硬鱼中，头部区域，特别是鳃，在渗透调节NaCl运输中起着关键作用，可能是通过位于氯细胞的机制。随着人们对氯离子细胞功能的具体机制的关注，两种经典的制备方法，完整的鱼和分离的、灌注的鳃，仍然是唯一可用的模型系统。然而，这两种制备都有严重的局限性，例如，由于它们不是平板，因此无法在短路电流技术实现的理想热力学条件下进行研究。本研究描述了鳉鱼和美洲海鸦头部皮肤特定区域的组织学和超微结构。这层皮肤位于包盖的内部，具有扁平的含氯细胞的上皮。在本研究中，用电子显微镜确定了该上皮中氯细胞的身份。虽然海鸦的眼上皮含有少量氯细胞，但适应池塘水、100%和200%人工海水的广盐鳉的眼上皮主要是氯细胞。值得注意的是，硬骨鱼鳃中氯化物细胞的含量从未超过10%。因此，鳉鱼的表皮可以作为研究氯细胞在广盐硬骨鱼体内适应性作用的模型。一项单独的电生理学研究表明，短路电流技术可以应用于这种皮肤。
In teleosts the head region, particularly the gills, plays the key role in osmoregulatory NaCl transport, presumably by mechanisms located in the chloride cell. As interest has focused on specific mechanisms of chloride cell function, two classical preparations, the intact fish and the isolated, perfused gill, have continued to serve as the only available model systems. However, both of these preparations have severe limitations, e.g., as they are not flat sheets, they cannot be studied readily under the ideal thermodynamic conditions achieved with the short‐circuit current technique. The present study describes the histology and ultrastructure of a particular area of skin in the head region of both killifish (Fundulus heteroclitus) and sea raven (Hemitripterus americanus). This skin lies on the inside of the operculum and possesses a flat epithelium containing chloride cells. In the present study the identity of the chloride cell in this epithelium was definitively established with the electron microscope. Although the opercular epithelium from the marine sea raven contains few chloride cells, that from the euryhaline killifish adapted to pond water, 100%‐, and 200% artificial seawater is predominately chloride cells. Significantly, the teleost gill has never been reported to contain more than 10% chloride cells. Thus the opercular skin of the killifish can serve as a model to study the adaptive role of chloride cells in euryhaline teleosts. A separate electrophysiological study has demonstrated that the short‐circuit current technique can be applied to this skin.