Differential expression of cytokines in subcutaneous and marrow fat of aging C57BL/6J mice
Differential expression of cytokines in subcutaneous and marrow fat of aging C57BL/6J mice
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DOI：
10.1016/j.exger.2009.05.009
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发表时间：
2009-09-01
影响因子：
3.9
通讯作者：
Duque, Gustavo
中科院分区：
文献类型：
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作者：
Gasparrini, Marco;Rivas, Daniel;Duque, Gustavo
增加骨髓脂肪生成在年龄相关性骨质流失的发病机制中起着致病作用，这可能与高细胞因子产生有关。在这项研究中，我们描述了骨髓（BM）脂肪细胞与皮下（SC）脂肪细胞中细胞因子表达的年龄相关变化。从幼年（4月龄）和老年（24月龄）雄性C57BL/6J中分离BM和SC脂肪细胞。提取总蛋白，使用细胞因子抗体阵列对96个细胞因子进行蛋白质组学分析。显示显著变化的蛋白质根据其在骨骼中的已知功能进行分组。我们发现53种细胞因子的表达存在显著的年龄诱导差异。与SC脂肪细胞相比，衰老的BM脂肪细胞表现出更促脂肪形成、抗成骨细胞形成和促凋亡的表型。这些数据表明，随着年龄的增长，BM脂肪细胞的毒性明显高于SC脂肪细胞。这些细胞因子，如果分泌出来，可能通过影响骨髓环境中的其他细胞，在与年龄相关的骨质流失的发病机制中发挥作用。(C) 2009爱思唯尔公司版权所有。
Increasing marrow adipogenesis plays a causative role in the pathogenesis of age-related bone loss that could be associated with high cytokine production. In this study, we characterized the age-related changes in cytokine expression by bone marrow (BM) adipocytes as compared with subcutaneous (SC) fat. BM and SC adipocytes were isolated from young (4 months) and old (24 months) male C57BL/6J. Total proteins were extracted and proteomic analysis of 96 cytokines was performed using a cytokine antibody array. Proteins showing a significant change were grouped according with their known function in bone. We found a significant age-induced difference in the expression of 53 cytokines. As compared with SC adipocytes, aging BM adipocytes showed a more pro-adipogenic, anti-osteoblastogenic and pro-apoptotic phenotype. These data suggest that, with aging, BM adipocytes become significantly more toxic than SC adipocytes. These cytokines, if secreted, could play a role in the pathogenesis of age-related bone loss by affecting other cells within the marrow milieu. (C) 2009 Elsevier Inc. All rights reserved.