ISOLATION AND PRELIMINARY CHARACTERIZATION OF THE TRANSFORMING GENE OF A HUMAN NEURO-BLASTOMA CELL-LINE
ISOLATION AND PRELIMINARY CHARACTERIZATION OF THE TRANSFORMING GENE OF A HUMAN NEURO-BLASTOMA CELL-LINE
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DOI：
10.1073/pnas.80.2.383
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
WIGLER, M
中科院分区：
文献类型：
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作者：
SHIMIZU, K;GOLDFARB, M;WIGLER, M
来自人神经母细胞瘤细胞系SK-N-SH的DNA能够在DNA介导的基因转移后诱导转化的NIH 3 T3细胞的病灶。利用大肠杆菌sup F基因的遗传选择，我们已经从小鼠细胞中分离出了负责致癌转化的人类序列。这些序列存在于所调查的所有人类DNA中，并且在SK-N-SH细胞中没有发现这些序列的总体重排。虽然与膀胱癌、肺癌和结肠癌细胞系中存在的其他两种人类转化基因明显不同，但所有三种转化基因序列可能是ras基因家族的相关成员。
DNA from the human neuroblastoma cell line SK-N-SH is capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer. Using genetic selection with the Escherichia coli sup F gene, we have isolated human sequences from mouse cells responsible for the oncogenic transformation. These sequences are present in all human DNAs surveyed and no gross rearrangements of these sequences are found in SK-N-SH cells. Although clearly distinct from two other human transforming genes present in bladder, lung, and colon carcinoma cell lines, all three transforming gene sequences may be related members of the ras gene family.