A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
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基于 Mizoroki-Heck 反应的从土壤中分离产衣康酸真菌的高通量筛选方法
DOI：
10.1016/j.heliyon.2019.e02048
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发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Aso Yuji
中科院分区：
文献类型：
--
作者：
Sano Mei;Kuroda Hikari;Ohara Hitomi;Ando Hiroshi;Matsumoto Keiji;Aso Yuji
在这项研究中，我们报告了一种新的方法的基础上的Mizoroki-Heck反应，然后碘试验的筛选产伊曲酸真菌从土壤中。该方法简便、快速，只需10 μL培养液，1.5 h内即可得到结果。发酵液中衣康酸的检出限为0.13 mM。这是首次报道利用偶联反应直接筛选产衣康酸真菌。
In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid in the cultures was 0.13 mM. This is the first report on the direct screening of itaconic acid-producing fungi using a coupling reaction.