A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp
A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp
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DOI：
10.1016/j.jip.2016.01.003
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发表时间：
2016-02-01
影响因子：
3.4
通讯作者：
Attasart, Pongsopee
中科院分区：
文献类型：
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作者：
Chimwai, Chaweewan;Tongboonsong, Punnee;Attasart, Pongsopee
单调对虾致密病毒（PmDNV）是泰国水产养殖业虾发育不良的主要原因之一。用含有PmDNV ns1和vp基因对应的灭活细菌的配方饲料喂养虾后，在预防和治疗实验中，虾肝胰脏PmDNV水平均显著降低。观察到PmDNV的显著减少约88%（预防性）和64%（治疗性），这表明该饲料在商业水产养殖中具有很大的应用潜力，可减少PmDNV相关的虾生长迟缓。(C) 2016 Elsevier Inc.版权所有。
Penaeus monodon densovirus (PmDNV) is one of the major causes of stunted shrimp in the aquaculture industry in Thailand. Significant reductions in levels of PmDNV as assessed by PCR analysis of shrimp hepatopancreas were seen in both prophylactic and curative experiments after feeding shrimp with a formulated diet containing mixed inactivated bacteria harboring dsRNAs corresponding to the PmDNV ns1 and vp genes. Significant reductions of approximately 88% (prophylactic) and 64% (curative) of PmDNV were observed, suggesting that this diet has a high potential for application in commercial aquaculture for reducing PmDNV associated stunted growth of shrimp. (C) 2016 Elsevier Inc. All rights reserved.