Electron microscopic and histochemical characterization of intra-arterial cushions of the rat and porcine uterine vascular bed.
Electron microscopic and histochemical characterization of intra-arterial cushions of the rat and porcine uterine vascular bed.
复制标题
大鼠和猪子宫血管床动脉内垫的电子显微镜和组织化学特征。
DOI：
10.1159/000145793
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
D. Farley
中科院分区：
文献类型：
--
作者：
P. Heidger;D. V. van Orden;D. Farley
扫描和透射电子显微镜研究，连同组织化学调查，进行了大鼠和猪动脉内垫从子宫血管床。在大鼠中，这些垫子的精细结构与先前在大鼠肾脏中描述的非常相似。垫子由修饰的平滑肌组成，环形布置在动脉分支入口周围的不完整的凸起带中。这些肌细胞作为衰减的过程在整个组织松散的PAS阳性基质中投射，并与从表面内皮细胞基部投射的薄内皮延伸物建立密切接触。扫描电子显微镜观察内皮表面上的沟引起的建议，这种接触可能介导的内皮表面拓扑结构的肌肉控制。在来自猪子宫动脉的类似垫子中，垫子的平滑肌组织更加致密，并且在垫子结构内径向而不是周向布置。猪垫的酶组织化学谱没有明显不同的正常血管平滑肌和内皮，这表明与邻近组织的代谢相似性的维护。这些研究阐明了最近报道的大鼠子宫血管床缺血和灌注过程中子宫动脉垫收缩和舒张的精细结构基础，从而阐明了它们在子宫血管流量调节中的功能作用。
Scanning and transmission electron microscopic studies, together with histochemical investigations, were conducted on rat and porcine intra-arterial cushions from the uterine vascular bed. In the rat, the fine structure of these cushions closely resembled that previously described in the rat kidney. The cushions were composed of modified smooth muscle, circularly disposed in an incomplete, raised band surrounding the entrance to arterial branches. These muscle cells projected as attenuated processes throughout the loosely organized, PAS-positive stroma, and established close contact with thin endothelial extensions projecting from the base of the surface endothelial cells. Scanning electron microscopic observations of furrows on the endothelial surface gave rise to the suggestion that such contacts might mediate muscular control of endothelial surface topology. In similar cushions from the pig uterine artery, the smooth muscle of the cushions was much more compactly organized, and was disposed radially, rather than circumferentially, within the cushion structure. The enzyme histochemical profile of porcine cushions did not differ appreciably from that of normal vascular smooth muscle and endothelium, suggesting the maintenance of a metabolic similarity with adjacent tissues. These studies clarify the fine-structural basis for recently reported contraction and relaxation of uterine artery cushions during ischemia and perfusion of the rat uterine vascular bed, and thus, for their functional role in the regulation of uterine vascular flow.