Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential
Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential
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中国牧马比氏肠细胞虫基因型及其人畜共患传播潜力
DOI：
10.1111/jeu.12308
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发表时间：
2016-09-01
影响因子：
2.2
通讯作者：
Zhang, Longxian
中科院分区：
文献类型：
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作者：
Qi, Meng;Wang, Rongjun;Zhang, Longxian
本研究采集了新疆中国自治区12组放牧马的粪便标本2 62份。对标本进行核糖体内转录间隔区(ITS)的聚合酶链式反应和序列分析。毕氏艾美耳球虫在马中的总患病率为30.9%(81/262)。不同年龄或性别的马之间的患病率没有显著差异。共鉴定出19个基因类型：15个已知基因(BEB6、CHG19、CM6、CM7、CM8、CS-1、CS-4、D、EPBA、EbpC、G、Horse1、Horse2、O和Peru8)和4个新基因(XJH1-XJH4)。此前在人类中发现了其中六种基因型：BEB6、D、EBPA、EbpC、O和Peru8。EbpC基因频率最高(21/81)，其次是EPBA(20/81)、BEB6(9/81)、CM6(4/81)、Horse1(4/81)、O(4/81)、G(3/81)、CHG19(2/81)、CM7(2/81)、Horse2(2/81)和XJH1(2/81)，其余8种基因各有1例。在系统发育分析中，除BEB6、CM7、Horse2、XJH1和XJH4外，有14个基因型(65/81，80.2%)属于第1类群，具有人畜共患潜力。毕氏艾美耳球虫的高度多样性及其人畜共患病潜力表明，放牧马是人畜共患传染病的潜在来源。
In present study, 262 fecal specimens were collected from 12 groups of grazing horses in the Xinjiang Uyghur Autonomous Region of China. The specimens were subjected to PCR and sequencing analyses of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi in horses was 30.9% (81/262). No significant differences in prevalence were observed between horses of different ages or sexes. Nineteen genotypes were identified: 15 known genotypes (BEB6, CHG19, CM6, CM7, CM8, CS‐1, CS‐4, D, EpbA, EbpC, G, horse1, horse2, O, and Peru8) and four new genotypes (XJH1–XJH4). Six of these genotypes were previously detected in humans: BEB6, D, EbpA, EbpC, O, and Peru8. Genotype EbpC was the most prevalent (21/81), followed by EpbA (20/81), BEB6 (9/81), CM6 (4/81), horse1 (4/81), O (4/81), G (3/81), CHG19 (2/81), CM7 (2/81), horse2 (2/81), and XJH1 (2/81), whereas the remaining eight genotypes were seen in one specimen each. In a phylogenetic analysis, 14 genotypes (65/81, 80.2%), excluding genotypes BEB6, CM7, horse2, XJH1, and XJH4, belonged to group 1, which have zoonotic potential. The high diversity in the E. bieneusi genotypes and their zoonotic potential suggest that grazing horses are a potential source of zoonotic infection in humans.