Effect of glucose, soya oil and glutamine on protein expression and mammalian target of rapamycin complex 1 pathway of jejunal crypt enterocytes in weaned piglets
Effect of glucose, soya oil and glutamine on protein expression and mammalian target of rapamycin complex 1 pathway of jejunal crypt enterocytes in weaned piglets
复制标题
葡萄糖、豆油和谷氨酰胺对断奶仔猪空肠隐窝肠细胞蛋白表达和雷帕霉素复合物1通路哺乳动物靶标的影响
DOI：
10.1017/s0007114519002629
复制
发表时间：
2019-10
期刊：
影响因子：
--
通讯作者：
Yin Yulong
中科院分区：
文献类型：
--
作者：
Xiong Xia;Lv Dinghong;Liu Yanhong;Song Min-ho;Zou Lijun;Xiao Dingfu;Yin Yulong
Abstract The present study was conducted to evaluate the effects of glucose, soya oil or glutamine on jejunal morphology, protein metabolism and protein expression of the mammalian target of rapamycin complex 1 (mTORC1) signalling pathway in jejunal villus or crypt compartment of piglets. Forty-two 21 d-weaned piglets were randomly allotted to one of the three isoenergetic diets formulated with glucose, soya oil or glutamine for 28 d. On day 14 or 28, the proteins in crypt enterocytes were analysed with isobaric tags for relative and absolute quantification and proteins involved in mTORC1 signalling pathway in villus or crypt compartment cells were determined by Western blotting. Our results showed no significant differences (P > 0·05) in jejunal morphology among the three treatments on day 14 or 28. The differentially expressed proteins mainly took part in a few network pathways, including antimicrobial or inflammatory response, cell death and survival, digestive system development and function and carbohydrate metabolism. On day 14 or 28, there were higher protein expression of eukaryotic initiation factor-4E binding protein-1 in jejunal crypt compartment of piglets supplemented with glucose or glutamine compared with soya oil. On day 28, higher protein expression of phosphor-mTOR in crypt compartment was observed in piglets supplemented with glucose compared with the soya oil. In conclusion, the isoenergetic glucose, soya oil or glutamine did not affect the jejunal morphology of piglets; however, they had different effects on the protein metabolism in crypt compartment. Compared with soya oil, glucose or glutamine may be better energy supplies for enterocytes in jejunal crypt compartment.
登录
查看更多内容
影响因子：
3.6
作者：
MAKKINK, CA;NEGULESCU, GP;VERSTEGEN, MWA
通讯作者：
VERSTEGEN, MWA
DOI：
10.1126/science.aag1417
发表时间：
2017-03-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Castellano BM;Thelen AM;Moldavski O;Feltes M;van der Welle RE;Mydock-McGrane L;Jiang X;van Eijkeren RJ;Davis OB;Louie SM;Perera RM;Covey DF;Nomura DK;Ory DS;Zoncu R
通讯作者：
Zoncu R
影响因子：
4.6
作者：
Kumar, Namit;Srivillibhuthur, Manasa;Verzi, Michael P.
通讯作者：
Verzi, Michael P.
影响因子：
3.3
作者：
Mendoza, S. M.;van Heugten, E.
通讯作者：
van Heugten, E.
影响因子：
3.5
作者：
Montagne, L;Crévieu-Gabriel, I;Lallès, JP
通讯作者：
Lallès, JP