Hydrogel fibers encapsulating human stem cells in an injectable calcium phosphate scaffold for bone tissue engineering.
Hydrogel fibers encapsulating human stem cells in an injectable calcium phosphate scaffold for bone tissue engineering.
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将人类干细胞封装在可注射磷酸钙支架中的水凝胶纤维用于骨组织工程
DOI：
10.1088/1748-6041/11/6/065008
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发表时间：
2016-11-04
期刊：
影响因子：
--
通讯作者：
Xu HH
中科院分区：
文献类型：
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作者：
Wang L;Wang P;Weir MD;Reynolds MA;Zhao L;Xu HH
人诱导多能干细胞（hiPSC）、人胚胎干细胞（hESC）和人脐带间充质干细胞（hUCMSC）是用于再生医学的令人兴奋的细胞来源。目前还没有关于将干细胞包裹在可注射磷酸钙骨水泥（CPC）支架内用于骨组织工程的长水凝胶纤维的报道。本研究的目的是：（1）开发一种新型的可注射CPC，其含有水凝胶纤维包裹细胞用于骨工程;（2）研究和比较hiPSC-MSCs、hESC-MSCs和hUCMSCs在可注射CPC中的细胞活力、增殖和成骨分化。包封干细胞的糊剂在小的注射力下是完全可注射的，并且与未注射的细胞相比，注射不会伤害细胞（p > 0.1）。干细胞-CPC构建体的机械性能比先前用于细胞递送的可注射聚合物和水凝胶的机械性能好得多。CPC中hiPSC-MSCs、hESC-MSCs和hUCMSCs在水凝胶纤维中具有良好的增殖和成骨分化能力。所有三种细胞类型均产生高碱性磷酸酶、runt相关转录因子、I型胶原和骨钙素表达（平均值± SD; n = 6）。细胞合成的矿物质随时间的延长而显著增加（p < 0.05），三种类型的细胞之间无显著差异（p > 0.1）。hiPSC-MSCs、hESC-MSCs和hUCMSCs在CPC中的矿化作用在14 d时是1 d时的13倍。总之，CPC支架中的所有三种类型的细胞（hiPSC-MSC、hESC-MSC和hUCMSC）均显示出用于骨组织工程的高潜力，并且具有细胞包封水凝胶纤维的新型可注射CPC构建体有希望用于增强牙科、颅面和整形外科应用中的骨再生。
Human induced pluripotent stem cells (hiPSCs), human embryonic stem cells (hESCs) and human umbilical cord mesenchymal stem cells (hUCMSCs) are exciting cell sources for use in regenerative medicine. There have been no reports on long hydrogel fibers encapsulating stem cells inside an injectable calcium phosphate cement (CPC) scaffold for bone tissue engineering. The objectives of this study were: (1) to develop a novel injectable CPC construct containing hydrogel fibers encapsulating cells for bone engineering, and (2) to investigate and compare cell viability, proliferation and osteogenic differentiation of hiPSC-MSCs, hESC-MSCs and hUCMSCs in injectable CPC. The pastes encapsulating the stem cells were fully injectable under a small injection force, and the injection did not harm the cells, compared with non-injected cells (p > 0.1). The mechanical properties of the stem cell–CPC construct were much better than those of previous injectable polymers and hydrogels for cell delivery. The hiPSC-MSCs, hESC-MSCs and hUCMSCs in hydrogel fibers in CPC had excellent proliferation and osteogenic differentiation. All three cell types yielded high alkaline phosphatase, runt-related transcription factor, collagen I and osteocalcin expression (mean ± SD; n = 6). Cell-synthesized minerals increased substantially with time (p < 0.05), with no significant difference among the three types of cells (p > 0.1). Mineralization by hiPSC-MSCs, hESC-MSCs and hUCMSCs in CPC at 14 d was 13-fold that at 1 d. In conclusion, all three types of cells (hiPSC-MSCs, hESC-MSCs and hUCMSCs) in a CPC scaffold showed high potential for bone tissue engineering, and the novel injectable CPC construct with cell-encapsulating hydrogel fibers is promising for enhancing bone regeneration in dental, craniofacial and orthopedic applications.