Insect Tissue Culture: Use of Blastokinetic Stage of Leafhopper Embryo
Insect Tissue Culture: Use of Blastokinetic Stage of Leafhopper Embryo
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DOI:
10.1126/science.144.3625.1465
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发表时间:
1964-06
期刊:
影响因子:
56.9
通讯作者:
H. Hirumi;K. Maramorosch
中科院分区:
文献类型:
--
作者:
H. Hirumi;K. Maramorosch
To find the proper material for cultivation in vitro of cells of leafhopper vectors of plant viruses, embryonic tissues of the six-spotted aster leaf-hopper (Macrosteles fascifrons) were tested during early developmental stages, during blastokinetic movement, and in late developmental stages. Growing cells were only obtained from the stage of blastokinetic movement. This stage can be determined visually in leafhopper eggs.