Insect Tissue Culture: Use of Blastokinetic Stage of Leafhopper Embryo

Insect Tissue Culture: Use of Blastokinetic Stage of Leafhopper Embryo
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DOI:
10.1126/science.144.3625.1465
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发表时间:
1964-06
期刊:
影响因子:
56.9
通讯作者:
H. Hirumi;K. Maramorosch
H. Hirumi;K. Maramorosch
中科院分区:
综合性期刊1区
文献类型:
--
作者:
H. Hirumi;K. Maramorosch

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为了寻找适合植物病毒载体叶蝉细胞体外培养的材料,对六斑紫菀叶蝉(Macrosteles fascifrons)的胚胎组织在发育早期、发育后期和发育后期进行了检测。生长细胞仅在胚动运动阶段获得。这个阶段可以在叶蝉卵中直观地确定。
To find the proper material for cultivation in vitro of cells of leafhopper vectors of plant viruses, embryonic tissues of the six-spotted aster leaf-hopper (Macrosteles fascifrons) were tested during early developmental stages, during blastokinetic movement, and in late developmental stages. Growing cells were only obtained from the stage of blastokinetic movement. This stage can be determined visually in leafhopper eggs.