Islet endothelial cells and pancreatic β-cell proliferation:: Studies in vitro and during pregnancy in adult rats
Islet endothelial cells and pancreatic β-cell proliferation:: Studies in vitro and during pregnancy in adult rats
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DOI：
10.1210/en.2005-0997
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发表时间：
2006-05-01
期刊：
影响因子：
4.8
通讯作者：
Carlsson, PO
中科院分区：
文献类型：
--
作者：
Johansson, M;Mattsson, G;Carlsson, PO
肿瘤和非肿瘤组织的生长都可能受到来自血管内皮的信号的影响。在本研究中，我们发现来自胰岛的纯化增殖内皮细胞可以通过分泌肝细胞生长因子（HGF）来刺激β细胞增殖。这种分泌可以由来自胰岛的可溶性信号诱导，如血管内皮生长因子- a （VEGF-A）和胰岛素。在怀孕期间，胰腺β细胞表现出高度可再生的生理增殖。我们发现，在怀孕动物中，胰岛内皮细胞增殖先于β细胞增殖。血管生长与内分泌细胞增殖密切相关，妊娠第15天胰岛内皮中HGF显著表达，即与β细胞增殖高峰相吻合。总之，我们的研究结果表明，成人胰岛内存在内皮-内分泌轴，这对成人β细胞增殖很重要。
The growth of both tumors and nonneoplastic tissues may be influenced by signals from the vascular endothelium. In the present investigation we show that purified proliferating endothelial cells from pancreatic islets can stimulate beta-cell proliferation through secretion of hepatocyte growth factor (HGF). This secretion could be induced by soluble signals from the islets, such as vascular endothelial growth factor-A (VEGF-A) and insulin. During pregnancy, the pancreatic beta-cells display a highly reproducible physiological proliferation. We show that islet endothelial cell proliferation precedes beta-cell proliferation in pregnant animals. Vascular growth was closely associated with endocrine cell proliferation, and prominent expression of HGF was observed in islet endothelium on d 15 of pregnancy, i.e. coinciding with the peak of beta-cell proliferation. In summary, our results suggest the existence of an endothelial-endocrine axis within adult pancreatic islets, which is of importance for adult beta-cell proliferation.