Differential quantification of cloned CVI988 vaccine strain and virulent RB-1B strain of Marek's disease viruses in chicken tissues, using real-time PCR
Differential quantification of cloned CVI988 vaccine strain and virulent RB-1B strain of Marek's disease viruses in chicken tissues, using real-time PCR
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DOI：
10.1016/j.rvsc.2010.08.002
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发表时间：
2011-08-01
影响因子：
2.4
通讯作者：
Nair, V. K.
中科院分区：
文献类型：
--
作者：
Baigent, S. J.;Smith, L. P.;Nair, V. K.
针对家禽马立克氏病的“金标准”疫苗是CVI 988/Rispens病毒，其在抗原性或遗传学上不容易与强毒马立克氏病疱疹病毒区分。CVI 988疫苗和强毒病毒的准确差异测量对于研究疫苗保护机制是重要的。CVI 988和强毒MDV毒株之间的最小序列差异限制了分子诊断方法如实时PCR在区分这些病毒方面的应用。使用细菌人工染色体（BAC）克隆的CVI 988病毒（其携带BAC载体序列代替U（s）2基因）允许使用分别靶向BAC载体序列和U（s）2基因的实时PCR测定法将其与强毒株进行差异定量。这些新的试验允许在马立克氏病的实验性疫苗接种-攻毒模型中研究血清1型疫苗病毒（克隆CVI 988）和攻毒病毒（RB-1B株）在个体鸡组织中的复制。(C)2010爱思唯尔有限公司版权所有。
The 'gold standard' vaccine against Marek's disease in poultry is the CVI988/Rispens virus, which is not easily distinguishable, antigenically or genetically, from virulent Marek's disease herpesvirus. Accurate differential measurement of the CVI988 vaccine and virulent viruses is important to investigate mechanisms of vaccinal protection. Minimal sequence differences between CVI988 and virulent MDV strains restrict the application of molecular diagnostic methods such as real-time PCR to distinguish between these viruses. The use of bacterial-artificial-chromosome (BAC) cloned CVI988 virus, which carries the BAC vector sequences in place of the U(s)2 gene, allows its differential quantification from virulent strains using real-time PCR assays that target the BAC vector sequence and the U(s)2 gene respectively. These novel assays allowed investigation of replication of both serotype-1 vaccine virus (cloned CVI988) and challenge virus (RB-1B strain) in tissues of individual chickens in an experimental vaccination-challenge model of Marek's disease. (C) 2010 Elsevier Ltd. All rights reserved.