Synchrotron FTIR Microspectroscopy of Single Natural Silk Fibers
Synchrotron FTIR Microspectroscopy of Single Natural Silk Fibers
复制标题
单根天然丝纤维的同步加速器 FTIR 显微光谱分析
DOI：
10.1021/bm2006032
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发表时间：
2011-09-01
影响因子：
6.2
通讯作者：
Chen, Xin
中科院分区：
文献类型：
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作者：
Ling, Shengjie;Qi, Zeming;Chen, Xin
利用同步辐射傅里叶变换红外光谱（S-FTIR）技术对一系列天然蚕丝（家蚕丝、野蚕丝和蜘蛛丝）的丝蛋白构象进行了研究。通过选择合适的孔径大小，我们获得了能够半定量分析蛋白质二级结构的高分辨率S-FTIR光谱。我们首次从S-FTTR中确定了天然单丝纤维中的β片层含量，在桑蚕丝、柞蚕丝和可食黄粉虫丝中分别为28 +/-4%、23 +/-2%和17 +/- 4%。不同蚕丝纤维中β-折叠含量的变化趋势与MID、拉曼和C-13 NMR测定的结果一致。我们的研究结果表明，S-FTIR显微光谱方法具有相当大的潜力，为单根天然丝纤维的研究。
Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTTR the beta-sheet content in a range of natural single silk fibers, 28 +/- 4, 23 +/- 2, and 17 +/- 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of beta-sheet content in different silk fibers from the current study accords quite well with published data determined by MID, Raman, and C-13 NMR. Our results indicate that the, S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers.