Biosensor based on glucose oxidase-nanoporous gold co-catalysis for glucose detection.
Biosensor based on glucose oxidase-nanoporous gold co-catalysis for glucose detection.
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DOI：
10.1016/j.bios.2014.11.037
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发表时间：
2015-04
影响因子：
12.6
通讯作者：
Chao Wu;Huihui Sun;Yufei Li;Xueying Liu;Xiaoyu Du;Xia Wang;P. Xu
中科院分区：
文献类型：
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作者：
Chao Wu;Huihui Sun;Yufei Li;Xueying Liu;Xiaoyu Du;Xia Wang;P. Xu
促进葡萄糖的电催化氧化对于葡萄糖生物传感器的设计至关重要。在本研究中，纳米多孔金（NPG）被选择用于葡萄糖氧化酶（GOx）固定和葡萄糖生物传感器制造，因为它具有开放、高导电性、生物相容性和互连的多孔结构，也有利于葡萄糖的电催化氧化。所得GOx/NPG/GCE生物电极表面的电化学反应归因于GOx和NPG的共催化作用。在循环伏安实验期间，在生物电极处观察到磷酸盐缓冲溶液中的表面限制反应。对于 50 μM 至 10 mM 范围内的大葡萄糖浓度，观察到线性响应，灵敏度为 12.1 μA mM−1cm−2，检测限低为 1.02 μM。此外，GOx/NPG/GCE生物电极对胆固醇、尿素、三丁酸甘油酯、抗坏血酸和尿酸具有很强的抗干扰能力，并且保质期长。对于人血清中葡萄糖的检测，GOx/NPG/GCE生物电极产生的数据与自动生化分析仪产生的数据非常吻合。这些独特的特性使 GOx/NPG/GCE 生物电极成为构建葡萄糖生物传感器的绝佳选择。
Promoting the electrocatalytic oxidation of glucose is crucial in glucose biosensor design. In this study, nanoporous gold (NPG) was selected for glucose oxidase (GOx) immobilization and glucose biosensor fabrication because of its open, highly conductive, biocompatible, and interconnected porous structure, which also facilitates the electrocatalytic oxidation of glucose. The electrochemical reaction on the surface of the resulting GOx/NPG/GCE bioelectrode was attributed to the co-catalysis effect of GOx and NPG. A surface-confined reaction in a phosphate buffer solution was observed at the bioelectrode during cyclic voltammetry experiments. Linear responses were observed for large glucose concentrations ranging from 50 μM to 10 mM, with a high sensitivity of 12.1 μA mM−1cm−2and a low detection limit of 1.02 μM. Furthermore, the GOx/NPG/GCE bioelectrode presented strong anti-interference capability against cholesterol, urea, tributyrin, ascorbic acid, and uric acid, along with a long shelf-life. For the detection of glucose in human serum, the data generated by the GOx/NPG/GCE bioelectrode were in good agreement with those produced by an automatic biochemical analyzer. These unique properties make the GOx/NPG/GCE bioelectrode an excellent choice for the construction of a glucose biosensor.