Cytogenetic effects in human exposure to arsenic
Cytogenetic effects in human exposure to arsenic
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DOI：
10.1016/s1383-5742(97)00009-4
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发表时间：
1997-06-01
影响因子：
5.3
通讯作者：
OstroskyWegman, P
中科院分区：
文献类型：
--
作者：
Gonsebatt, ME;Vega, L;OstroskyWegman, P
本文研究了生活在同一地理区域、社会经济地位相似但饮用水中无机砷暴露程度不同的农村人群砷暴露的细胞遗传学效应。圣安娜居民（饮用水中砷含量为408.17 μ g/l）被认为是暴露个体，纳扎列诺居民（29.88 μ g/l）被认为是对照组。研究人员采集了志愿者的血液和尿液样本。通过问卷调查和体格检查，确定了研究参与者过去和现在的砷暴露、健康和营养状况以及砷皮肤病变的存在。在全血淋巴细胞培养中研究了第一分裂中期染色体畸变的频率和类型，同时在口腔粘膜和尿液样本中获得的脱落上皮细胞中研究了微核（MN）的存在。测定尿液中总砷（TAs）含量、无机砷（IAs）的相对比例以及代谢产物一甲基胂酸（MMA）和二甲基胂酸（DMA）的含量。暴露个体淋巴细胞中染色单体和等染色单体缺失的频率显著增加，口腔和尿上皮细胞中MN缺失的频率显著增加。男性比女性更容易受到影响，并且在皮肤病变的个体中发现了更多的微核口腔细胞。观察到的细胞遗传损伤类型证明砷是一种致裂/非致生致癌物。
The cytogenetic effects of arsenic exposure were studied among rural populations that live in the same geographical area and have similar socioeconomic status, but different degree of exposure to inorganic arsenic (As) via drinking water. A group of inhabitants of Santa Ana (408.17 mu g/l of As in drinking water) were considered the exposed individuals and a group of inhabitants of Nazareno (29.88 mu g/l) were considered as controls. Blood and urine samples were obtained from volunteers. Past and current exposure, health, and nutritional status as well as the presence of arsenic skin lesions were ascertained in study participants through questionnaires and physical examination. The frequencies and types of chromosomal aberrations in first-division metaphases were studied in whole blood lymphocyte cultures while the presence of micronuclei (MN) was studied in exfoliated epithelial cells obtained from the oral mucosa and from urine samples. Total arsenic (TAs) content, and the relative proportions of inorganic arsenic (IAs), and the metabolites monomethylarsonic (MMA) and dimethylarsinic (DMA) acid were determined in urine samples. Exposed individuals showed a significant increase in the frequency of chromatid and isochromatid deletions in lymphocytes and of MN in oral and urinary epithelial cells. Males were more affected than females, and a higher number of micronucleated oral cells were found among those individuals with skin lesions. The type of cytogenetic damage observed gives evidence of arsenic as a clastogenic/aneugenic carcinogen.