Different mitogenic activity of soluble and insoluble staphylococcal protein A (SPA).
Different mitogenic activity of soluble and insoluble staphylococcal protein A (SPA).
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发表时间：
1978-09
期刊：
影响因子：
6.4
通讯作者：
S. Romagnani;Alberto Amadori;M. Giudizi;R. Biagiotti;Enrico Maggi;Mario Ricci
中科院分区：
文献类型：
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作者：
S. Romagnani;Alberto Amadori;M. Giudizi;R. Biagiotti;Enrico Maggi;Mario Ricci
研究了高度纯化的外周血和扁桃体人淋巴细胞群体对SPA和葡萄球菌菌株科万I（StaCw）的反应。从外周血中分离纯化的T淋巴细胞通过E-玫瑰花结在体外不能响应StaCw。在可溶性SPA存在下，来自扁桃体的高度纯化的B细胞群体没有显示出任何增殖反应。在StaCw存在下，向来自人扁桃体的高度纯化的B细胞悬浮液中加入浓度增加的自体T淋巴细胞不会诱导胸苷摄取的任何增加。然而，即使添加了丝裂霉素处理的T淋巴细胞，它也能够恢复B细胞培养物对可溶性SPA的显着增殖反应。高纯度外周血T淋巴细胞对可溶性SPA的低反应性可通过加入自体丝裂霉素处理的B细胞来增强，而纯化的T淋巴细胞对StaCw的无反应性不受影响。SPA偶联Sepharose珠的促有丝分裂活性与可溶性SPA不同，而与StaCw相似。这些数据有力地表明，不溶性SPA是一种T细胞非依赖性的B细胞有丝分裂原在人，而可溶性SPA，如PWM，发挥其对B细胞的活性，只有在T细胞的存在。
The response to SPA and Staphylococcus strain Cowan I (StaCw) of highly purified populations of peripheral blood and tonsil human lymphocytes was investigated. Purified T lymphocytes isolated from perpheral blood by E-rosetting were unable to respond in vitro to StaCw. Highly purified B-cell populations from tonsils did not show any proliferative response in the presence of soluble SPA. The addition to highly purified B-cell suspensions from human tonsils of increasing concentrations of autologous T lymphocytes did not induce any increase of thymidine uptake in the presence of StaCw. However, it was able to restore a marked proliferative response of the B-cell cultures to soluble SPA, even though mitomycin-treated T lymphocytes were added. The low response of highly purified peripheral blood T lymphocytes to soluble SPA could be potentiated by the addition of autologous mitomycin-treated B cells, whereas the unresponsiveness of purified T lymphocytes to StaCw was not affected. Mitogenic activity of SPA coupled to Sepharose beads was different from that of soluble SPA and paralleled that of StaCw. These data strongly suggest that insoluble SPA is a T-cell-independent B-cell mitogen in man, whereas soluble SPA, like PWM, exerts its activity on B cells only in the presence of T cells.