Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry
Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry
复制标题
通过亲水相互作用色谱结合四重飞行时间串联质谱法对整个哺乳期的猪乳寡糖进行注释
DOI：
10.1002/elps.201500471
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发表时间：
2016-06-01
期刊：
影响因子：
2.9
通讯作者：
Yin, Heng
中科院分区：
文献类型：
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作者：
Cheng, Likun;Xu, Qingsong;Yin, Heng
猪在畜牧业中发挥着重要作用。作为母猪和仔猪之间的纽带，母乳对新生仔猪的健康至关重要。游离乳低聚糖是哺乳动物乳汁中一类重要的生物活性物质。然而，对猪MO（PMO）的组成和功能知之甚少。在这项研究中，亲水相互作用色谱与四极杆飞行时间串联质谱（HILIC-Q-TOF MS）系统耦合用于分析PMO。在三个品种的母猪中共鉴定出41种不同的PMO。PMO高度唾液酸化（约30%），岩藻糖基化PMO（1-3%）监测水平较低。最丰富的寡糖是三糖（Hex 3），占总PMO丰度的50%以上。自由MOs谱的比较揭示了个体间和不同品种母猪间的异质性和变异性，但品种间MOs的变异有限，甚至小于个体间的变异。初乳中大多数PMO含量较高，泌乳早期下降，但少数PMO含量最终上升。不同的低聚糖在哺乳期有不同的模式。总体而言，这些观察显示了更详细的PMO库，并将有助于探索PMO对仔猪健康的影响。
Swine plays a significant role in livestock agriculture. As a linkage between sows and piglets, porcine milk is crucial for the health of newborn piglets. Free milk oligosaccharides (MOs) are kinds of important bioactive substance in mammalian milk. However, little is known about the component and function of the porcine MOs (PMOs). In this study, a hydrophilic interaction chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry (HILIC‐Q‐TOF MS) system was utilized to profile the PMOs. Forty‐one distinct PMOs were identified totally in three breeds of sows. The PMOs were highly sialylated (∼30%) and fucosylated PMOs (1–3%) were monitored at low levels. The most abundant oligosaccharide was a trisaccharide (Hex3) which contributed over 50% of the total PMOs abundance. Comparison of free MOs profiles revealed heterogeneity and variations among individuals and different breeds of sows, however, the MOs variation among breeds was limited even minor than that among individuals. Furthermore, most PMOs contents were higher in colostrum and decreased in the early lactation, but a few kinds increased at last. Different oligosaccharides had different patterns during lactation. Overall, these observations showed a more detailed PMOs library and would contribute to the exploration of influence of PMOs on piglets’ health.