Anti-Infection Tissue Engineering Construct Treating Osteomyelitis in Rabbit Tibia
Anti-Infection Tissue Engineering Construct Treating Osteomyelitis in Rabbit Tibia
复制标题
抗感染组织工程构建治疗兔胫骨骨髓炎
DOI：
10.1089/ten.tea.2012.0262
复制
发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Xu, Jianzhong
中科院分区：
文献类型：
--
作者：
Xing, Junchao;Hou, Tianyong;Xu, Jianzhong
骨缺损并发感染仍然是骨科手术的一大挑战，初次修复的植骨往往失败率较高。组织工程骨和抗生素输送系统研究领域的快速进展为解决这一问题带来了希望。在此，我们评估了可注射抗感染组织工程结构的局部抗感染和成骨潜力，该结构包括纤维蛋白凝胶支架和万古霉素海藻酸盐珠（Vanco-AB）形成复合材料，用于治疗兔胫骨骨缺损的慢性骨髓炎。建立兔胫骨感染性骨缺损模型。然后，将构建的复合材料植入近端胫骨干骺端的骨缺损中，其中包含间充质干细胞和 Vanco-AB 的不同组合。通过放射学评估、组织病理学观察和微生物培养来确定移植物的体内抗感染和局部成骨能力。结果表明，由纤维蛋白凝胶支架和 Vanco-AB 组成的可注射抗感染组织工程构建体可有效消灭细菌。移植后1个月和3个月时，放射学评估和微生物学检查表明，Vanco-AB持续释放的抗生素显着降低了骨髓炎的Norden评分，对细菌的存在产生了阴性结果，并减少了骨髓炎的复发。同时，一阶段植入的组织工程结构促进了局部骨修复和重建，与对照组（无Vanco-AB）相比，表现出更明显的成骨潜力。总之，本研究实现了感染性骨缺损的初步修复，从而为慢性骨髓炎常见的感染性骨缺损提供了一种替代治疗策略。
Bone defect complicated by infection remains a major challenge in orthopedic surguries, and bone grafting for primary repair often associates with high failure rate. The rapid progress in the research spectrums of tissue-engineered bone and antibiotic delivery systems bring hope to solve this issue. Herein, we evaluated the local anti-infective and osteogenic potential of an injectable anti-infection tissue-engineered construct, which includes a fibrin gel scaffold and vancomycin alginate beads (Vanco-AB) to form composites, in the treatment of chronic osteomyelitis with bone defect in rabbit tibia. The infected bone defect model of rabbit tibia was established. Then, the bone defects in the proximal tibial metaphysis were implanted with the constructed composites, containing different combinations of mesenchymal stem cells and Vanco-AB. The in vivo capacities of anti-infection and local osteogenesis of the grafts were determined using radiographic assessment, histopathological observation, and microorganism cultures. Results showed that the injectable anti-infection tissue-engineered construct, comprising a fibrin gel scaffold and Vanco-AB led to efficient eradication of bacteria. At 1 and 3 months after transplantation, the radiographic assessment and microbiological examination demonstrated that the sustained antibiotic release by Vanco-AB significantly decreased the Norden scores of osteomyelitis, generated negative results for the presence of bacteria, and reduced the relapse of osteomyelitis. Meanwhile, tissue-engineered construct implanted in one-stage promoted local bone repair and reconstruction, and it exhibited more apparent osteogenic potential, compared to the control group (without Vanco-AB). In conclusion, the current study achieved the primary repair of bone defect with infection, thus providing an alternative treatment strategy for infected bone defect, which occurs commonly in chronic osteomyelitis.