Identification and Characterization of a Human CD5+ Pre-Naive B Cell Population
Identification and Characterization of a Human CD5+ Pre-Naive B Cell Population
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DOI：
10.4049/jimmunol.0803391
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发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Lipsky, Peter E.
中科院分区：
文献类型：
--
作者：
Lee, Jisoo;Kuchen, Stefan;Lipsky, Peter E.
我们已经鉴定了在人外周血中循环的独特的幼稚前B细胞群，其表现出介于过渡和幼稚B细胞之间的中间表型。与人移行B细胞一样，这些细胞表达CD 5，但与移行和幼稚B细胞相比，具有中等密度的CD 38、CD 10、CD 9和ABCB 1转运蛋白。这些幼稚前B细胞占循环人CD 5（+）B细胞的大部分。重要的是，CD 5（+）pre-naive B细胞可以在体外被诱导分化为具有naive表型的细胞。CD 5（+）pre-naive B细胞对BCR刺激和CD 40连接仅表现出部分应答，并且比naive B细胞经历更多的自发性凋亡和细胞死亡，而BAFF/BLyS（属于TNF家族的B细胞活化因子）与naive B细胞相比并没有增强它们的存活。相比之下，CD 5+前幼稚B细胞执行与幼稚B细胞相当的某些功能，包括分化成浆细胞的能力和充当APC的能力。值得注意的是，在系统性红斑狼疮患者的外周血中发现CD 5+前幼稚B细胞的比例增加。这些结果已经确定了一个独特的中间人幼稚B细胞的发展在外周血和紊乱的稳态系统性红斑狼疮患者。免疫学杂志，2009，182：4116-4126.
We have identified a distinct pre-naive B cell population circulating in human peripheral blood that exhibits an intermediate phenotype between transitional and naive B cells. Like human transitional B cells, these cells express CD5 but have intermediate densities of CD38, CD10, CD9, and the ABCB1 transporter compared with transitional and naive B cells. These pre-naive B cells account for a majority of circulating human CD5(+) B cells. Importantly, CD5(+) pre-naive B cells could be induced to differentiate into cells with a naive phenotype in vitro. CD5(+) pre-naive B cells show only partial responses to BCR stimulation and CD40 ligation and undergo more spontaneous apoptosis and cell death than do naive B cells, whereas BAFF/BLyS (B cell-activating factor belonging to the TNF family) did not enhance their survival compared with naive B cells. In contrast, CD5+ pre-naive B cells carry out certain functions comparable to naive B cells, including the capacity to differentiate into plasma cells and the ability to function as APCs. Notably, an increased proportion of CD5+ pre-naive B cells were found in peripheral blood of patients with systemic lupus erythematosus. These results have identified a unique intermediate in human naive B cell development within the peripheral blood and derangements of its homeostasis in patients with systemic lupus erythematosus. The Journal of Immunology, 2009, 182: 4116-4126.