Cell cluster formation in degenerate lumbar intervertebral discs is associated with increased disc cell proliferation
Cell cluster formation in degenerate lumbar intervertebral discs is associated with increased disc cell proliferation
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DOI：
10.3109/03008200109005650
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发表时间：
2001-01-01
影响因子：
2.9
通讯作者：
Roberts, S
中科院分区：
文献类型：
--
作者：
Johnson, WEB;Eisenstein, SM;Roberts, S
健康人的椎间盘细胞相对较少，且分布稀疏。然而，椎间盘退变的一个特征是出现细胞团簇，特别是在受损区域。这些星团是如何形成的目前尚不清楚。我们检查了切除的病理人椎间盘以寻找细胞增殖的证据。对椎间盘切片进行增殖细胞核抗原（PCNA）和增殖相关Ki-67抗原的免疫染色。在退变椎间盘内观察到PCNA免疫阳性细胞，通常但不是唯一的细胞团。Ki-67抗原免疫阳性的细胞比PCNA免疫阳性的细胞少，但在退变椎间盘的集群中也经常观察到类似的情况。相比之下，这些标记物的免疫阳性在退变程度较低的椎间盘或未观察到细胞团的椎间盘区域并不常见。这些观察结果表明，椎间盘细胞增殖与椎间盘退变有关，并且可能是细胞簇形成的原因。
Healthy human intervertebral discs contain relatively few cells and these are sparsely distributed. A characteristic feature of disc degeneration, however, is the appearance of cell clusters, particularly in damaged areas. How these clusters form is currently unknown. We have examined excised pathological human discs for evidence of cell proliferation. Disc sections were immunostained for the proliferating cell nuclear antigen (PCNA) and the proliferation-associated Ki-67 antigen. PCNA immunopositive cells were observed within degenerate discs, commonly though not exclusively, in cell clusters. Cells immunopositive for the Ki-67 antigen were less prevalent than those for PCNA, but similarly were observed frequently within clusters in degenerate discs. In contrast, immunopositivity for these markers was not common in less degenerate discs or in areas of the disc where cell clusters were not observed. These observations suggest that disc cell proliferation is associated with disc degeneration and is the likely cause of cell cluster formation.