Immunochemical evidence for the presence of advanced glycation end products in human lens proteins and its positive correlation with aging.
Immunochemical evidence for the presence of advanced glycation end products in human lens proteins and its positive correlation with aging.
复制标题
DOI：
10.1016/s0021-9258(19)50004-7
复制
发表时间：
1992-05
期刊：
影响因子：
--
通讯作者：
N. Araki;N. Ueno;B. Chakrabarti;Y. Morino;S. Horiuchi
中科院分区：
文献类型：
--
作者：
N. Araki;N. Ueno;B. Chakrabarti;Y. Morino;S. Horiuchi
蛋白质与还原糖的长时间孵育通过一系列反应进行，包括早期产物到具有荧光、棕色和交联的晚期糖基化终产物。这些变化统称为美拉德反应，被认为是糖尿病并发症和衰老过程中的因素。早期产物已在体内得到证实，但晚期糖基化终产物在体内存在的证据有限。我们试图通过制备和使用针对这些终产物的多克隆和单克隆抗体来提供免疫化学证据（Horiuchi，S.，Araki，N.，和Morino，Y.（1991）J. Biol. Chem. 266，7329-7332）作为探针来鉴定和定量人透镜晶状体蛋白中的此类化合物。两种抗体均不与婴儿晶状体提取物反应，但成人晶状体组分显示出显著的反应性，与透镜年龄相关。我们的研究结果提供了第一个免疫化学证据表明，人类透镜晶体蛋白含有晚期糖基化终产物，这些产物随着组织年龄的增加而增加。
Prolonged incubation of protein with reducing sugar proceeds through a series of reactions involving early stage products to the advanced glycation end products with fluorescence, brown color, and cross-linking. Known collectively as the Maillard reaction, these changes have been suggested as factors in diabetic complications and the aging process. The early stage products have been demonstrated in vivo, but evidence for the presence in vivo of the advanced glycation end products has been limited. We sought to provide immunochemical evidence by the preparation and use of polyclonal and monoclonal antibodies to these end products (Horiuchi, S., Araki, N., and Morino, Y. (1991) J. Biol. Chem. 266, 7329-7332) as probes to identify and quantitate such compounds in human lens crystallins. Neither of the antibodies reacted with extracts from infant lenses, but fractions from adult lenses showed a significant reactivity, correlating with lens age. Our findings provide the first immunochemical evidence that human lens crystallins contain advanced glycation end products and that these products increase with tissue age.