BMP2-induced gene profiling in dental epithelial cell line.
BMP2-induced gene profiling in dental epithelial cell line.
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DOI：
10.2152/jmi.55.216
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发表时间：
2008-08
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影响因子：
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通讯作者：
Keiko Miyoshi;Hideya Nagata;Taigo Horiguchi;K. Abe;Ivan Arie Wahyudi;Y. Baba;H. Harada;T. Noma
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文献类型：
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作者：
Keiko Miyoshi;Hideya Nagata;Taigo Horiguchi;K. Abe;Ivan Arie Wahyudi;Y. Baba;H. Harada;T. Noma
牙齿发育受上皮-间充质相互作用及其相互分子信号的调节。骨形态发生蛋白2（BMP 2）是牙齿发育的诱导因子之一。为了分析BMP 2对成釉细胞分化（成釉）的分子机制，我们使用大鼠牙上皮细胞系HAT-7进行了微阵列分析。在证实BMP 2可以激活HAT-7细胞中的经典BMP-Smads信号传导后，我们分析了BMP 2对14，815个基因表达的影响并对其进行了分析。BMP-2处理HAT-7细胞24 h后，73个基因表达上调，28个基因表达下调。功能分类显示，18%的上调基因是存在于釉质器官中的ECM/粘附分子。此外，我们研究了几种分化标志物在牙齿上皮细胞的四个细胞系，包括内釉上皮细胞（成釉细胞），中间层，网状层，和外釉上皮细胞的表达。结果表明，BMP 2可能诱导至少两种不同的细胞系标志物，包括在HAT-7细胞中表达的BMP拮抗剂，这表明BMP 2可以通过BMP信号促进釉质形成。
Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (BMP2) is known as one of the inducers for tooth development. To analyze the molecular mechanisms of BMP2 on ameloblast differentiation (amelogenesis), we performed microarray analyses using rat dental epithelial cell line, HAT-7. After confirming that BMP2 could activate the canonical BMP-Smads signaling in HAT-7 cells, we analyzed the effects of BMP2 on 14,815 gene expressions and profiled them. Seventy-three genes were up-regulated and 28 genes were down-regulated by BMP2 treatment for 24 hours in HAT-7 cells. Functional classification revealed that 18% of up-regulated genes were ECM/adhesion molecules present in the enamel organ. Furthermore, we examined the expression of several differentiation markers in dental epithelial four cell-lineages including inner enamel epithelium (ameloblasts), stratum intermedium, stratum reticulum, and outer enamel epithelium. The results indicated that BMP2 might induce at least two different cell-lineage markers including a BMP antagonist expressed in HAT-7 cells, suggesting that BMP2 could accelerate amelogenesis via BMP signaling.