FACTORS AFFECTING THE ION-PAIR CHROMATOGRAPHY OF WATER-SOLUBLE VITAMINS
FACTORS AFFECTING THE ION-PAIR CHROMATOGRAPHY OF WATER-SOLUBLE VITAMINS
复制标题
DOI：
10.1016/s0021-9673(00)94458-3
复制
发表时间：
1988-06-17
期刊：
影响因子：
--
通讯作者：
TARUMOTO, T
中科院分区：
文献类型：
--
作者：
DONG, MW;LEPORE, J;TARUMOTO, T
系统地评价了七种水溶性维生素在反相柱上分离的控制因素。研究的因素包括移动的相成分和柱参数。数据显示，含有己烷磺酸盐（5 mM）、甲醇（15%）、乙酸（1%）和三乙胺（0.10-0.13%）的移动的相在几个C8和C18柱下产生了优异的分离。降低移动的相中的甲醇浓度可提高早期洗脱峰的分离度，而三乙胺水平可控制硫胺素的峰形和保留时间。评价了开发的液相色谱（LC）分离的分析精密度、耐用性和灵敏度。发现LC分离的稳定性在4个月内令人满意。
Factors controlling the separation of seven water-soluble vitamins on reversed-phase columns were systematically evaluated. Factors studied include both mobile phase constituents and column parameters. Data showed that a mobile phase containing hexanesulfonate (5 mM), methanol (15%), acetic acid (1%), and triethylamine (0.10-0.13%) yielded excellent separations with several C8 and C18 columns. Lowering the methanol concentration in the mobile phase enhanced the resolution of early eluting peaks, while the triethylamine levels controlled the peak shape and retention of thiamine. The analytical precision, robustness, and sensitivity of the developed liquid chromatographic (LC) separation were evaluated. The stability of the LC separation was found to be satisfactory for over a 4-month period.