Mechanisms related to the genotoxicity of particles in the subway and from other sources
Mechanisms related to the genotoxicity of particles in the subway and from other sources
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DOI：
10.1021/tx7003568
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发表时间：
2008-03-01
影响因子：
4.1
通讯作者：
Moeller, Lennart
中科院分区：
文献类型：
--
作者：
Karlsson, Hanna L.;Holgersson, Asa;Moeller, Lennart
流行病学研究清楚地表明了空气中颗粒物对健康的负面影响。人们接触到来自各种来源的颗粒，例如柴油和木材的燃烧以及轮胎-道路磨损产生的颗粒。对某些人群来说，另一个重要的来源是暴露于地铁系统中的颗粒物。我们最近报道说，这些颗粒更遗传毒性时，相比，其他几种颗粒类型。本研究的目的是进一步调查和比较地铁颗粒物和其他来源的颗粒物的毒性，以及调查地铁颗粒物遗传毒性背后的一些机制。这是通过比较地铁颗粒和来自街道的颗粒、纯轮胎-道路磨损颗粒以及来自木材和柴油燃烧的颗粒引起线粒体去极化和形成细胞内活性氧（ROS）的能力来完成的。此外，将遗传毒性和引起氧化应激的能力与磁铁矿颗粒进行了比较，因为磁铁矿颗粒是地铁颗粒的主要成分。得出的结论是，地铁颗粒和街道颗粒和颗粒从木材和柴油燃烧引起线粒体去极化。因此，破坏线粒体的能力并不是地铁颗粒高遗传毒性的唯一解释。地铁颗粒也形成细胞内ROS。这种效应可以部分解释为什么地铁颗粒与其他颗粒相比显示出如此高的遗传毒性。然而，遗传毒性不能用主要成分磁铁矿、水溶性金属或细胞内动员的铁来解释。遗传毒性最有可能是由引起氧化应激的高反应性表面引起的。
Negative health effects of airborne particles have clearly been shown in epidemiological studies. People get exposed to particles from various sources such as the combustion of, for example, diesel and wood and also from particles arising from tire-road wear. Another source of importance for certain populations is exposure to particles in subway systems. We recently reported that these particles were more genotoxic when-compared to that of several other particle types. The aim of this study was to further investigate and compare the toxicity of subway particles and particles from other sources as well as investigate some mechanisms behind the genotoxicity of subway particles. This was done by comparing the ability of subway particles and particles from a street, pure tire-road wear particles, and particles from wood and diesel combustion to cause mitochondrial depolarization and to form intracellular reactive oxygen species (ROS). Furthermore, the genotoxicity and ability to cause oxidative stress was compared to magnetite particles since this is a main component in subway particles. It was concluded that the subway particles and also street particles and particles from wood and diesel combustion caused mitochondrial depolarization. The ability to damage the mitochondria is thus not the only explanation for the high genotoxicity of subway particles. Subway particles also formed intracellular ROS. This effect may be part of the explanation as to why subway particles show such high genotoxicity when compared to that of other particles. Genotoxicity can, however, not be explained by the main component, magnetite, by water-soluble metals, or by intracellular mobilized iron. The genotoxicity is most likely caused by highly reactive surfaces giving rise to oxidative stress.