Proteome-wide identification of lysine 2-hydroxyisobutyrylation reveals conserved and novel histone modifications in Physcomitrella patens.
Proteome-wide identification of lysine 2-hydroxyisobutyrylation reveals conserved and novel histone modifications in Physcomitrella patens.
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赖氨酸 2-羟基异丁酰化的全蛋白质组鉴定揭示了小立碗藓中保守且新颖的组蛋白修饰
DOI：
10.1038/s41598-017-15854-z
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发表时间：
2017-11-14
影响因子：
4.6
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Yu Z;Ni J;Sheng W;Wang Z;Wu Y
蛋白质赖氨酸2-羟基异丁酰化（Khib）是一种新发现的动物和酵母细胞中的翻译后修饰。先前的研究表明，组蛋白Khib参与雄性细胞分化，并在动物染色质功能的调节中发挥关键作用。然而，关于植物中蛋白质Khib的信息仍然有限。在这项研究中，使用特异性抗体和LC-MS/MS方法，我们在小立碗藓的3，001个蛋白质中鉴定了11，976个Khib位点。生物信息学分析表明，这些Khib修饰蛋白参与了广泛的分子功能和细胞过程，并显示出不同的亚细胞定位。此外，通过比较人、小鼠和展布对虾组蛋白的Khib位点，发现展布对虾组蛋白H3和H4中存在保守位点，而H1、H2 A和H2 B中存在新位点。这是第一份关于植物中Khib翻译后修饰的报告，该研究提供了小立碗藓组蛋白和非组蛋白蛋白中Khib位点的全面概况。
Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly identified post-translational modification found in animal and yeast cells. Previous research suggested that histone Khib is involved in male cell differentiation and plays a critical role in the regulation of chromatin functions in animals. However, information regarding protein Khib in plants is still limited. In this study, using a specific antibody and LC-MS/MS methods, we identified 11,976 Khib sites in 3,001 proteins in Physcomitrella patens. The bioinformatics analysis indicated that these Khib-modified proteins were involved in a wide range of molecular functions and cellular processes, and showed diverse subcellular localizations. Furthermore, an comparism of Khib sites in histone proteins among human, mouse and P. patens found conserved sites in the H3 and H4 histone proteins and novel sites in H1, H2A and H2B histone proteins in P. patens. This is the first report on Khib post-translational modifications in plants, and the study provides a comprehensive profile of Khib sites in histone and non-histone proteins in Physcomitrella patens.
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影响因子：
5.1
作者：
Horst NA;Reski R
通讯作者：
Reski R
影响因子：
64.5
作者：
Huang H;Sabari BR;Garcia BA;Allis CD;Zhao Y
通讯作者：
Zhao Y
影响因子：
14.8
作者：
Dai, Lunzhi;Peng, Chao;Zhao, Yingming
通讯作者：
Zhao, Yingming
新型组蛋白翻译后修饰的蛋白质组学表征。
影响因子：
3.9
作者：
Arnaudo AM;Garcia BA
通讯作者：
Garcia BA
影响因子：
4.4
作者：
Picchi, Gisele F. A.;Zulkievicz, Vanessa;de Godoy, Lyris M. F.
通讯作者：
de Godoy, Lyris M. F.