Two‐dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines
Two‐dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines
复制标题
大豆胞囊线虫 Heterodera gigines 的二维蛋白质组参考图谱
作者：
X. Chen;M. Macdonald;Farooq Khan;W. Garrett;B. Matthews;S. Natarajan
构建了大豆胞囊线虫的 2-DE 参考图谱。用胰蛋白酶进行凝胶内消化后，随后通过 LC-MS/MS 鉴定出代表 426 种蛋白质的 803 个点。具有注释功能的蛋白质通过基因本体论进一步分类。结果表明，参与代谢、发育和生物调节过程的蛋白质最为丰富。
2‐DE reference maps of Heterodera glycines were constructed. After in‐gel digestion with trypsin, 803 spots representing 426 proteins were subsequently identified by LC‐MS/MS. Proteins with annotated function were further categorized by Gene Ontology. The results showed that proteins involved in metabolic, developmental and biological regulation processes were the most abundant.