Cloning and sequence analysis of hepatic lipase (HL) and liportein lipase (LPL) gene in Orange-Spotted Grouper(Epinephelus coioides)
Cloning and sequence analysis of hepatic lipase (HL) and liportein lipase (LPL) gene in Orange-Spotted Grouper(Epinephelus coioides)
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发表时间：
2010
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通讯作者：
Huang Zhi-dong
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作者：
Huang Zhi-dong
为研究肝脂肪酶（HL）和脂质蛋白脂肪酶（LPL）基因在脂质代谢中的分子机制及其分子进化关系，采用RT-PCR和RACE方法从橙斑石斑鱼（Epinephelus coioides）肝脏中获得了HL和LPL的全长cDNA序列。序列分析表明，橙斑石斑鱼HL基因全长2261bp，编码区1485bp，编码494个氨基酸。LPL全长2191bp，编码区1548bp，编码515个氨基酸。序列分析表明，HL和LPL保守氨基酸序列的一致性表明，哺乳动物在进化后期的相似性均低于硬骨鱼。HL和LPL氨基酸序列在哺乳动物和鱼类中相对保守。系统发育分析表明，与中华鲟相比，橙斑石斑鱼的氨基酸序列更接近大口黑鲈和红鲷。本研究为HL和LPL氨基酸序列的同一性提供了可靠的证据，有助于探讨与脂质代谢有关的生理机制，有助于预防海洋鱼类脂质代谢疾病。
To research the molecular mechanism of hepatic lipase(HL) and liportein lipase(LPL) genes in lipid metabolism as well as their molecular evolutional relationship,the full-length cDNA sequences encoding HL and LPL were obtained from the liver of orange-spotted grouper(Epinephelus coioides) by RT-PCR and RACE methods.The sequence analysis showed that the complete cDNA sequence of HL of orange-spotted grouper was 2261bp in length,containing a coding region of 1485bp(encoding 494 amino acids).And the complete cDNA sequence of LPL was 2191bp in length,containing a coding region of 1548bp(encoding 515 amino acids).Sequence analysis reveals that,the identity of conserved amino acid sequence of HL and LPL revealed the similarity of mammals was both lower than teleosts later in the evolution.the identities of HL and LPL amino acid sequence among mammals and fishes were relatively conserved.Phylogenetic analyses revealed that,the deduced amino acid sequence of the orange-spotted grouper was more similar to largemouth bass and red sea bream than Chinese Sturgeon.This research provides reliable evidences about the identity of amino acid sequence of HL and LPL and is useful for investigating physiology mechanism in relation to lipid metabolism and help to prevent beforehand lipid metabolism diseases in marine fish.