Pathogenicity and Host-Parasite Relationships of Heterodera cruciferae in Cabbage
Pathogenicity and Host-Parasite Relationships of Heterodera cruciferae in Cabbage
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DOI：
10.1094/pdis-07-12-0699-re
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发表时间：
2013-03-01
期刊：
影响因子：
4.5
通讯作者：
Castillo, P.
中科院分区：
文献类型：
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作者：
Sasanelli, N.;Vovlas, N.;Castillo, P.
Sasanelli，N.，Vovlas，N.，Trisciuzzi，N.，Canalapiedra-Navarrete，C.，Palomares-Rius，J.E.，和Castillo，P.2013。甘蓝十字异尖线虫的致病性及寄主关系。植物Dis.97：333-338。在意大利南部塔兰托省卡斯特拉内塔市的大片空地上，观察到发育迟缓的卷心菜(Lupini)与一种形成包囊的线虫严重侵袭土壤有关。根据成熟的包囊、雄性和第二期幼虫的形态特征和核糖体DNA的分子分析(28S的D2到D3扩展片段和内部转录间隔区[ITS]1区)来鉴定该物种。ITS1序列信息支持十字异源线虫的同源性，也显示出与异源线虫群的其他物种高度相似的特征，包括异源线虫H.goettingiana、线虫H.carotae和麻黄线虫H.urticae。线虫通过诱导典型的多核合胞体，成功地在甘蓝根中建立了永久的取食部位，引起了根皮层、内皮层、中柱和维管柱的细胞变化。合体细胞质颗粒状致密，有大小不一的空泡和肥大的核仁。在致病性测试中，甘蓝植株的生长也受到了抑制。土壤中线虫初始种群密度与地上部分植株重量之间的关系符合Seinhorst方程。甘蓝对十字花科蔬菜地上部植株重量的耐受极限为1.50个单位的鸡蛋加1/2s/cm(3)的土壤。在线虫种群密度(PI)=单位卵加土壤量(J)/cm(3)时，株高的最小相对值(M)为0.71。线虫的最大繁殖率(Pf/PI)是初始种群密度的4.6倍，即8个单位的卵+2s/cm(3)的土壤。
Sasanelli, N., Vovlas, N., Trisciuzzi, N., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., and Castillo, P. 2013. Pathogenicity and host parasite relationships of Heterodera cruciferae in cabbage. Plant Dis. 97:333-338. Stunted cabbage ('Lupini') associated with severe soil infestations by a cyst-forming nematode were observed in large patches of open fields in Castellaneta, province of Taranto, southern Italy. Morphological traits based on mature cysts, males, and second-stage juveniles (J2s) and molecular analysis of ribosomal DNA (D2 to D3 expansion segments of 28S and internal transcribed spacer [ITS] 1 region) were used to identify the species. ITS1 sequence information supported the identity of Heterodera cruciferae, also showing a high degree of similarity to other species of the Heterodera Goettingiana group, including H. goettingiana, H. carotae, and H. urticae. Nematodes successfully established permanent feeding sites in cabbage roots which caused cellular alterations in the root cortex, endodermis, pericycle, and vascular cylinder by inducing typical multinucleate syncytia. Syncytial cytoplasm was granular and dense, with variously sized vacuoles and hypertrophied nuclei with nucleoli. Cabbage plant growth was also reduced in pathogenicity tests. The relationship between the initial nematode population density in soil and shoot plant weight was well described by the Seinhorst's equation. Tolerance limits with respect to shoot plant weight of cabbage to H. cruciferae was estimated as 1.50 units of eggs plus J2s/cm(3) of soil. The minimum relative value (m) for plant height was 0.71 at an initial nematode population density of (Pi) >= 64 units of eggs plus J2s/cm(3) of soil. The maximum nematode reproduction rate (Pf/Pi) was 4.6 times that of the initial population density of 8 units of eggs plus J2s/cm(3) of soil.