Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration.
Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration.
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DOI：
10.1016/j.ejpb.2008.02.011
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发表时间：
2008-08
期刊：
影响因子：
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通讯作者：
Wei Wei-Wei;Lianyan Wang;Lan Yuan;Xiao-da Yang;Z. Su;G. Ma
中科院分区：
文献类型：
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作者：
Wei Wei-Wei;Lianyan Wang;Lan Yuan;Xiao-da Yang;Z. Su;G. Ma
壳聚糖微球在医药领域具有很大的应用潜力。本研究采用石拉苏多孔玻璃(SPG)膜乳化技术制备了大小均匀的戊二醛交联壳聚糖微球(CG微球)。基于大小均匀和自发荧光的特点，建立了一种新的检测系统，可同时观察和定量大鼠口服3种不同直径(2.1、7.2和12.5μm)的CG微球。该系统结合了扫描电子显微镜(SEM)、激光扫描共聚焦显微镜(LSCM)和流式细胞仪技术，具有操作简单、直观、重复性好、灵敏度高等优点。首次详细研究了三种不同粒径的微球口服后在胃肠道的生物黏附、在胃肠道的吸收、在全身组织中的分布以及在网状内皮系统中的生物降解性。不同粒径的CG微球在生物黏附、吸收和分布方面表现出不同的命运，而不同的微球在RES中的位置不同，其生物降解性也不同。这些初步结果为CG微球的临床应用提供了一条更好的途径。
Chitosan microspheres have a great potential in pharmaceutical application. In this study, uniform-sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) were prepared by Shirasu Porous Glass (SPG) membrane emulsification technique. Based on the characterizations of uniform size and autofluorescence, it was possible to develop a new detecting system for observing and quantifying the CG microspheres in rats with three different diameters (2.1, 7.2 and 12.5μm) synchronously after oral administration. This system was a combination of scanning electron microscopy (SEM), laser scanning confocal microscope (LSCM) and flow cytometer technique, which showed the advantages of being simple, intuitionistic, repeatable and sensitive. After oral administration of three kinds of particles with different diameters, bioadhesion in gastrointestinal tract, absorption in gastrointestinal tract, distribution in systemic tissues, and biodegradation in reticuloendothelial system (RES) were studied firstly in detail. The CG microspheres showed different fates in bioadhesion, absorption and distribution according to their diameters, while the biodegradation also varied due to the different locations in RES. These original results would indicate a better way for the CG microspheres in the clinical application.