Kinetochore-microtubule interaction during S phase in Saccharomyces cerevisiae
Kinetochore-microtubule interaction during S phase in Saccharomyces cerevisiae
复制标题
DOI：
10.1101/gad.449407
复制
发表时间：
2007-12-15
影响因子：
10.5
通讯作者：
Tanaka, Tomoyuki U.
中科院分区：
文献类型：
--
作者：
Kitamura, Etsushi;Tanaka, Kozo;Tanaka, Tomoyuki U.
在出芽酵母酿酒酵母中，称为纺锤体极体 (SPB) 的微管组织中心嵌入核膜中，核膜在整个细胞周期中保持完整（闭合有丝分裂）。在细胞周期的大部分时间（包括 G1 和 M 期）中，动粒通过微管与 SPB 相连；然而，在染色体复制过程中微管相互作用是持续维持还是短暂破坏一直是一个争论的话题。在这里，我们发现着丝粒与微管分离 1-2 分钟，并在早期 S 期远离纺锤体极。这些脱离和移位事件是由着丝粒 DNA 复制引起的，从而导致着丝粒分解。不久之后，着丝粒重新组装，导致它们被微管重新捕获。我们还展示了动粒随后如何通过微管向极地运输。我们的研究为闭合有丝分裂 S 期的着丝粒-微管相互作用和着丝粒复制提供了新的见解。
In the budding yeast Saccharomyces cerevisiae, microtubule-organizing centers called spindle pole bodies (SPBs) are embedded in the nuclear envelope, which remains intact throughout the cell cycle (closed mitosis). Kinetochores are tethered to SPBs by microtubules during most of the cell cycle, including G1 and M phases; however, it has been a topic of debate whether microtubule interaction is constantly maintained or transiently disrupted during chromosome duplication. Here, we show that centromeres are detached from microtubules for 1-2 min and displaced away from a spindle pole in early S phase. These detachment and displacement events are caused by centromere DNA replication, which results in disassembly of kinetochores. Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are subsequently transported poleward by microtubules. Our study gives new insights into kinetochore-microtubule interaction and kinetochore duplication during S phase in a closed mitosis.