In Vivo Release from Cerebral Cortex of [14C]Glutamate Synthesized from [U‐14C]Glutamine
In Vivo Release from Cerebral Cortex of [14C]Glutamate Synthesized from [U‐14C]Glutamine
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[U-14C]谷氨酰胺合成的[14C]谷氨酸从大脑皮层体内释放
DOI：
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发表时间：
1983
影响因子：
4.7
通讯作者：
Henry F. Bradford
中科院分区：
文献类型：
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作者：
C. Thanki;D. Sugden;A. J. Thomas;Henry F. Bradford
摘要：清醒、不受约束和行为正常的动物（在感觉运动皮层上植入灌注插管）用于研究输注[U-14 C]谷氨酰胺标记去极化刺激释放的谷氨酸和其他氨基酸的能力。检测到[14 C]谷氨酸盐的自发背景释放。tityustoxin（1 μM）可增加该值。在诱发释放期间，谷氨酸的比放射性增加了8倍。还检测到[14 C]天冬氨酸，并显示释放增加，但对去极化反应的特异性标记未增加。诱发γ-氨基丁酸（GABA）释放，但仅检测到少量[14 C]GABA。谷氨酰胺在刺激期间表现出对感觉运动皮层的摄取率增加，表明通过谷氨酰胺酶加速分解。
Abstract: Awake, unrestrained, and behaviourally normal animals with superfusion cannulae implanted over the sensorimotor cortex were used in a study of the capacity of infused [U‐14C]glutamine for labelling glutamate and other amino acids released by depolarising stimuli. A spontaneous background release of [14C]glutamate was detected. This was increased by tityustoxin (1 μM). The specific radioactivity of glutamate increased eightfold during the evoked‐release period. [14C]Aspartate was also detected and showed increased release, but not increased specific labelling, in response to depolarisation. Evoked γ‐aminobutyric acid (GABA) release occurred but only small amounts of [14C]GABA were detected. Glutamine showed increased rates of uptake to the sensorimotor cortex during stimulation periods, suggesting an accelerated breakdown via glutaminase.