Localization and proliferation of lymphatic vessels in the tympanic membrane in normal state and regeneration.
Localization and proliferation of lymphatic vessels in the tympanic membrane in normal state and regeneration.
复制标题
正常状态下鼓膜内淋巴管的定位和增殖及再生。
DOI：
10.1016/j.bbrc.2013.09.054
复制
发表时间：
2013
影响因子：
3.1
通讯作者：
Peti-Peterdi,Janos
中科院分区：
文献类型：
--
作者：
Miyashita,Takenori;Burford,JamesL;Hong,Young-Kwon;Gevorgyan,Haykanush;Lam,Lisa;Mori,Nozomu;Peti-Peterdi,Janos
我们澄清了定位的淋巴管在鼓膜和增殖的淋巴管再生后，鼓膜穿孔，通过使用整体安装成像的Prox1 GFP小鼠鼓膜。在张力部，淋巴管袢环绕锤骨柄和鼓室环。除此之外，在正常鼓膜的张力部中未观察到淋巴管袢。锤骨柄周围的淋巴管袢与松弛部和鼓张肌周围的淋巴管袢相连。在松弛部发现许多淋巴管环路。鼓膜穿孔后，在张力部观察到丰富的淋巴管再生，这些再生的淋巴管在第7天从锤骨周围的淋巴管延伸。这些结果表明，位点特异性淋巴管在鼓膜中起着重要作用。
We clarified the localization of lymphatic vessels in the tympanic membrane and proliferation of lymphatic vessels during regeneration after perforation of the tympanic membrane by using whole-mount imaging of the tympanic membrane of Prox1 GFP mice. In the pars tensa, lymphatic vessel loops surrounded the malleus handle and annulus tympanicus. Apart from these locations, lymphatic vessel loops were not observed in the pars tensa in the normal tympanic membrane. Lymphatic vessel loops surrounding the malleus handle were connected to the lymphatic vessel loops in the pars flaccida and around the tensor tympani muscle. Many lymphatic vessel loops were detected in the pars flaccida. After perforation of the tympanic membrane, abundant lymphatic regeneration was observed in the pars tensa, and these regenerated lymphatic vessels extended from the lymphatic vessels surrounding the malleus at day 7. These results suggest that site-specific lymphatic vessels play an important role in the tympanic membrane.